摘要
本研究对甘蔗愈伤分化过程进行了观察 ,并对分化过程中的内源激素进行了测定、分析。结果表明 :甘蔗愈伤分化分胚性细胞发生、分化苗发生和绿苗长高等过程。与此同时 ,内源激素发生一系列的变化 ,IAA的合成在胚性细胞大量发生时达到峰值 ,分化苗大量发生时 ,CTK达到峰值 ,GA1 +3 ,在分化 2 0 d达到峰值 ,它的合成与分化苗的长高密切相关 ,分化后期 ,各种内源激素含量下降。
This paper reported studies on the differentiation of sugarcane callus and the changes of endogenous hormones in the course of differentiation.The endogenous hormones change with the differentiation of callus.The compound of IAA is related with the occurrence of embryonic cell and it come to the maximum when there are plenty of embryo.The differentiated shoots occur at the maximum content of CTK,but the composure of GA 1+3 is related with the growth height of differentiated shoots,the peak content of GA 1+3 is in the 20th day after the differentiation. The contents of all endogenous hormones reduce in 20 days after the differentiation.
出处
《种子》
CSCD
北大核心
2003年第1期10-11,共2页
Seed
基金
云南省"九五"攻关资助项目
关键词
愈伤组织
甘蔗
绿苗分化
内源激素
组织培养
Sugarcane Shoots diffierentiation Endogenous hormones Tissue culture
