摘要
利用紫外分光光度法研究并确定了从发酵菌体中提取辅酶Q10的工艺条件,即90℃回流30 min和石油醚萃取等,结果表明,采用皂化法提取、紫外分光光度法测定发酵菌体中辅酶Q10含量,不但具有较好的重现性和稳定性,而且其平均回收率高达(101.36±3.4)％,RSD为3.1％(n=3).
UV spectrophometer was used to detect CoQ10 in this paper, and we got the most suitable extracting procedure as following: Wet culture was distilled at 90℃ for 30 min, and extracted with petroleum ether twice. The result showed that this method had good repeatibility and stability, and the average recovery of CoQ10 was (101. 36 ± 3.4) % , with RSD( n = 3) of 3. 1 % .
出处
《无锡轻工大学学报（食品与生物技术）》
CSCD
北大核心
2003年第2期59-62,共4页
Journal of Wuxi University of Light Industry
关键词
辅酶Q10
提取
测定
coenzyme Q10( CoQ10 )
extraction
measurement
