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兔移核重构胚再程序化相关基因片段的分离与鉴定 被引量：1

Isolation and Identification of Reprogramming Genes Related to the Development of the Rabbit Reconstructed Embryos
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摘要 用单个植入前胚胎mRNA差别显示方法 (SinglePreimplantationEmbryonicmRNADifferentialDisplayReversePolymeraseReaction ,SPEDDRT PCR) ,以家兔移核重构发育至 2细胞、8细胞时期的胚胎及囊胚作为起始材料。研究家兔移核重构胚再程序化相关基因的表达。获得了 2 5个与再程序化相关的基因片段 ,完成了对所有片段的克隆、序列分析 ,其中 5个反向Northern证实的片段在从MⅡ到囊胚的发育阶段中呈现特异性表达的特点。这项研究为再程序化相关基因全长的分离以及功能研究奠定了良好的基础。 By the method of single preimplantation embryos differential display polymerase chain reaction (SPEDDRT PCR),25 reprogramming cDNA fragments were obtained from single 2 cell,8 cell embryos and blastula. After cloning and sequencing, five of them were identified by reverse Northern and characterized with stage specific expression during reconstructed embryo development. This results will help to isolate full length reprogramming genes and study their function during embryonic development.
作者 郁卫东 李文雍 王玉阁 杨立新 刘桂生 杜淼 陈清轩
机构地区 中国科学院遗传和发育生物学研究所中国科学院研究生院
出处 《生物工程学报》 CAS CSCD 北大核心 2003年第1期30-34,共5页 Chinese Journal of Biotechnology
基金 国家重大基础研究发展规划 (973 )资助 (No .2 0 0 0 0 1610 7)~~
关键词 兔移核重构胚 再程序化 相关基因片段 分离 鉴定 cloning, preimplantation embryos, single embryos mRNA differential display, reprogramming
分类号 S813 [农业科学—畜牧学]
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参考文献22

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同被引文献22

  • 1张弛,陈受宜.利用DDRT-PCR技术分析在盐胁迫下水稻耐盐突变体中特异表达的基因[J].中国科学（B辑）,1995,25(8):840-847. 被引量：28
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生物工程学报
2003年 第1期

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