摘要
采用单引物RT PCR扩增的方法 ,从新疆野生植物盐角草 (Salicorniaeuropaea)中克隆获得了 1.7kb的cDNA片段 ,经过测序和序列分析 ,发现基因片段包含了orf2 5基因完整的读码框架。采用序列同源性分析方法 ,结果显示新疆盐角草orf2 5基因与甜菜线粒体同源性高达 98% ,与烟草线粒体同源性达到 95 % ,与小麦同源性为 92 % ,与玉米同源性为 88% ,表明orf2 5基因在植物中是高度保守的一种基因 ,同时说明野生植物盐角草中也存在与农作物相似的雄性不育相关基因 ,orf2 5基因编码的功能性蛋白可能在影响植物雄性不育改良作物品种方面具有重要的意义。
The gene orf25 encodes functional protein that may play an important role in plant fertility control in nature. To clone the orf25 from Salicornia europaea Xinjiang into a T vector, a single designed primer was used to amplify 1.7kb cDNA fragment with RT PCR. Sequence analysis reveals that the cloned fragment contains entire orf25 coding region with 98%, 95%, 92% and 88% identity to that of orf25 from Beta vulgaris, Nicotiana, wheat and maize mitochondrion, respectively. This analysis suggests that orf25 gene is highly conserved in terms of evolution in plant; and it also suggests that wild plant Salicornia europaea contain a male sterility gene similar to crops that is of great importance in improvement of the breed of crop.
出处
《生物工程学报》
CAS
CSCD
北大核心
2003年第1期120-123,共4页
Chinese Journal of Biotechnology
基金
国家科技攻关项目资助 (No .2 0 0 1BA90 1A3 2 )~~
