摘要
【目的】为了明确中哈边境区域黑腿星翅蝗Calliptamus barbarus Costa的遗传多样性,阐明其区域性发生的遗传机制。【方法】测定中哈边境新疆边境区域阿勒泰、塔城、博乐、伊犁等和哈萨克斯坦境内黑腿星翅蝗5个种群100个体的COⅠ基因全长序列(1 540 bp),利用DnaSP 5.0,MEGA 6.0和Arlequin3.5等软件分析种群遗传多样性与分化情况。【结果】共获得100条COⅠ基因序列,在5个地理种群中发现122个变异位点,包含69个单倍型,其中1个单倍型为4个种群所共享。种群间的遗传距离范围为0.001-0.086。总群体单倍型多样性指数H_d=0.987,各地理种群单倍型多样性介于0.973-0.995之间,总群体核苷酸多样性P_i=0.008 4,总群体的遗传分化系数G_(st)=0.004 5,总群体固定系数F_(st)=0.014 2,总基因流N_m=4.61。中性检验(Tajima's D=-1.553 3,P>0.05;Fu’s Fs=-3.732 4,P>0.05)结果表明中哈边境黑腿星翅蝗种群在较近的历史上没有出现群体扩张。单倍型网络图和NJ单倍型系统树结果一致,博乐种群与其他种群分化较明显,AMOVA分子变异分析结果表明中哈边境不同地理种群黑腿星翅蝗的遗传分化主要来自种群内部(98.56%),种群间变异水平很低(1.44%)。不同地理种群的遗传距离与地理距离间的相关性不显著。各单倍型散布在不同地理种群中,未形成明显的系统地理结构。【结论】中哈边境区域不同地理种群黑腿星翅蝗基因交流频繁,遗传分化程度低。
[Objectives]To investigate the genetic diversity of Calliptamus barbarus in the border area between China and Kazakhstan,and reveal the molecular mechanism underlying serious regional outbreaks of this pest.[Methods]Specimens of C.barbarus were collected from 5 different geographic populations(ALT,TC,BL,YL and Kazakhstan)on the border between China and Kazakhstan.A total of 100 full-length sequences of the mitochondrial cytochrome oxidase subunitⅠ(COⅠ)gene(1 540 bp)were obtained,sequenced,and compared,using DnaSP 5.0 and Arlequin 3.5 software.[Results]About 122 mutations were found,including 69 haplotypes,one of which was shared by all 4 populations.The genetic distance between populations ranged from 0.001 to 0.086 and the total haplotype diversity index(Hd)was 0.987.The diversity of haplotypes among different geographic populations ranged from 0.973 to 0.995.The total nucleotide diversity(Pi),total genetic differentiation coefficient(Gst),total fixed coefficient(Fst),and total gene flow(Nm)were 0.008 4,0.004 5,0.014 2 and 4.61,respectively.Neutrality test results(Tajima′s D=-1.553 3,P>0.05;Fu’s Fs=-3.732 4,P>0.05)indicated that C.barbarus populations on the border between China and Kazakhstan have not recently expanded.The information obtained from the haplotype network and a neighbour-joining phylogenetic tree were consistent,and the differentiation of the BL population from other populations was obvious.The genetic difference between populations(1.44%)is much less than the genetic variation within populations,indicating that geographic distance has no obvious effect on the genetic distance between different populations.No obvious geographic structure is apparent in a phylogenetic tree and a haplotype network based on haplotypes of the different geographic populations.[Conclusion]There is frequent gene exchange between different geographic populations of C.barbarus in the border area between China and Kazakhstan and genetic differentiation of this species in this region is consequently low.
作者
麦季玮
徐叶
施燚
王添歆
ROMAN Jashenko
赵伟
王晗
MAI Ji-Wei;XU Ye;SHI Yi;WANG Tian-Xin;ROMAN Jashenko;ZHAO Wei;WANG Han(School of Life Sciences,Lanzhou University,Lanzhou 730000,China;International Research Center of Cross-border Pest Management in Central Asia,College of Life Sciences,Xinjiang Normal University,Urumqi 830054,China;Al-Farabi Kazakh National University,Almaty 050038,Kazakhstan)
出处
《应用昆虫学报》
CAS
CSCD
北大核心
2019年第2期263-272,共10页
Chinese Journal of Applied Entomology
基金
国家重点研发计划(2016YFE0203100)
兰州大学大创项目(20180180060)
