摘要
目的改进制备犬心肌梗死模型的方法,形成透壁性心肌梗死。方法健康成年杂种犬16只,随机分为对照组(n=8)和实验组(n=8);对照组结扎左冠状动脉前降支,实验组结扎左冠状动脉前降支及第一对角支,建立心肌梗死模型。6周后,进行心脏大体形态及组织病理学观察。结果犬术后6周,对照组存活6只,存活率为75.0%,实验组存活5只,存活率为62.5%,两组存活率无统计学差异(P=0.590)。对照组存活的6只犬中,有2只犬大体及组织病理观察均见明显的心肌梗死区,而另外4只犬大体观察未见明显的心肌梗死区,组织病理观察可见梗死灶;实验组存活的5只犬大体及组织病理观察均可见明显的心肌梗死区;两组形成透壁性心肌梗死的犬只数有统计学差异(P=0.022)。结论结扎犬左冠状动脉前降支及第一对角支,能形成透壁性心肌梗死,是一种制备心肌梗死模型的有效可靠方法。
Aim To improve the method of making myocardial infarction models in dogs and to form transmural myocardial infarction. Methods 16 healthy adult mongrel dogs were randomly divided into control group( n = 8) and experimental group( n = 8). The myocardial infarction was established. In control group,the left anterior descending coronary artery was ligated. While in experimental group,the left anterior descending coronary artery and first diagonal branch were ligated. After 6 weeks,cardiac general morphology and histopathology were observed. Results After 6weeks,there were 6 dogs survived in control group and 5 dogs survived in experimental group,and the survival rates were75. 0% and 62. 5% respectively. There was no statistically significant difference at survival rate in two groups( P =0. 590). Of the survived 6 dogs in the control group,there were 2 dogs that myocardial infarction could be seen clearly by cardiac general morphology and histopathology; Of the other 4 dogs,there was no myocardial infarction by cardiac general morphology,but micro-infarction was observed by histopathology. Of the survived 5 dogs in the experimental group,myocardial infarction could be observed clearly by cardiac general morphology and histopathology in all dogs. There was significant difference in the dog number of transmural myocardial infarction in two groups( P = 0. 022). Conclusion The ligation of the left anterior descending coronary artery and first diagonal branch in dog can form transmural myocardial infarction,which is an effective and reliable method for preparation of myocardial infarction model.
出处
《中国动脉硬化杂志》
CAS
北大核心
2015年第10期1001-1004,共4页
Chinese Journal of Arteriosclerosis
关键词
心肌梗死
动物模型
犬
Myocardial Infarction
Animal Model
Dog
