摘要
目的 探究CpG 2006对大鼠实验性牙移动及牙槽骨改建的影响。方法 选择雄性Wistar大鼠,建立上颌牙齿移动模型,左侧作为实验组,于第一磨牙颊黏膜下局部注射CpG 2006,右侧作为对照组,对应部位注射等量PBS。每3天给药一次,14 d后处死大鼠,测量大鼠上颌第一磨牙移动距离,并拍摄X线片,采用Real-time PCR技术检测牙周组织中成骨、破骨及炎症相关因子表达,通过HE染色检测CpG 2006对各脏器的影响。结果 大鼠实验侧第一磨牙移动距离明显小于对照侧(P<0.05),实验侧牙周组织中Runx2、SP7、OCN mRNA表达水平高于对照侧(P<0.01),而IL-1、IL-6、TNF-αmRNA表达水平明显低于对照侧(P<0.01),双侧Nfatc、CTSK、MMP9 mRNA表达水平无统计学差异,脏器组织学检测未见明显异常。结论 CpG 2006可通过促进牙周组织成骨活化来调控牙齿移动,并降低该过程中炎症因子的表达。
Objective To investigate the influence of CpG 2006 on tooth movement and alveolar bone remodeling in rats.Methods Male Wistar rats were chosen.Orthodontic tooth movement devices were installed at both maxillary of each rat,and the left side was chosen to be the treatment lateral,while the right side the control lateral.CpG 2006 or PBS was injected under the buccal mucosa of the maxillary first molar.Injection repeated every 72 h to all the animals sacrificed 14 days later.The migration distance of the molar was measured depending on specimen and X-ray.The expression levels of osteoclast and inflammatory related factors were detected by Real-time PCR.HE staining was used for detecting the effects of CpG 2006 on the organ.Results The migration distance of the molar in treatment lateral was smaller and the expression levels of IL-1,IL-6,TNF-ɑmRNA were lower than the control lateral,while the expression levels of Runx2,SP7,OCN mRNA were higher than the control lateral,and there were no differences among the expression levels of Nfatc,CTSK,MMP9 mRNA between the two laterals.No pathological changes were found in their organs.Conclusion CpG 2006 can regulate the distance of tooth movement by promoting the osteogenic activation of periodontal tissue,and reduce the expression of inflammatory factors during tooth movement.
作者
秦甜甜
于文雯
汪洋
刘宇妍
黄蕾
周雪纯
孙新华
QIN Tiantian;YU Wenwen;WANG Yang;LIU Yuyan;HUANG Lei;ZHOU Xuechun;SUN Xinhua(Department of Orthodontics,Hospital of Stomotology,Jilin University,Changchun130021,China)
出处
《口腔医学》
CAS
2019年第9期769-773,共5页
Stomatology
基金
吉林省省级医药健康产业发展专项资金项目(20170311032YY)
关键词
CPG
2006
成骨
炎症
正畸牙移动
大鼠
CpG 2006
osteogenesis
inflammation
orthodontic tooth movement
rats
