摘要
通过比较离体叶片接种、切茎接种、游动孢子液灌根接种等 3种不同接种方法对 8个辣椒品种 (品系 )的影响 ,发现这 3种方法均能用于鉴定辣椒对辣椒疫霉菌的抗性。其中离体叶片接种法是从田间采集叶片进行室内鉴定 ,能够在短时间内检测多个品种 ,并且对植株的生长无明显影响 ,最适合对种质资源稀少的品种进行抗疫病鉴定 ;切茎接种法操作简单 ,可以测量病斑的长度 ,反映病斑的扩展速度 ,适宜对抗性进行量化 ;游动孢子液灌根接种法基本上模拟田间病害的发生过程 ,最能反映品种的真实抗性 ,但工作量大 ,操作较复杂。研究者可以根据自己的研究目的 ,采取适当的接种方法对辣椒品种进行抗疫病鉴定。
Effects of different inoculation methods, A) leaf in vitro inoculation, B) cut-off stem inoculation, and C) drench with zoosperm inoculation on eight pepper varieties were compared. Results showed that the three methods were all effective ways to test the resistance to pepper phytophthora blight. In method A, leaves were collected from field and detected in laboratory. Therefore it could test many varieties at short time, and it had no effect on plant growth. The method is especially adapts to testing rare varieties. Method B operated simply and could measure the length of lesion, and indicate its extending speed. It is suitable to test resistance quantitatively. Method C simulated the field condition, and it could reflect the true resistance of each variety. But it was so complex that the operator had to do lots of work. Therefore researchers can choose suitable inoculation method according to different conditions to evaluate the resistance of different pepper varieties.
出处
《中国蔬菜》
北大核心
2003年第2期16-18,共3页
China Vegetables
基金
国家自然科学基金项目 (39870 45 3)
农业部"948"引进项目(2 0 10 2 3)
农业部蔬菜遗传与生理重点开放实验室经费资助
关键词
辣椒疫病
接种方法
抗病性鉴定
Phytophthora blight, Inoculation method, Resistance evaluation
