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Using Subtracted AFLP to Efficiently Mark an Alien Chromosome Fragment in Wheat Background 被引量：7

利用减法AFLP有效标记小麦中的外源染色体片段(英文)
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摘要 为解决AFLP中样品间共同片段对多态性片段识别的干扰 ,作者建立了一种“减法AFLP”标记技术 ,并运用这种技术成功对小麦中的外源染色体片段进行了标记。运用该技术 ,样品间的共同扩增片段显著减少 ,在非变性聚丙稀酰胺凝胶上带型差异十分明显 ,多态性片段很容易分辨。其真实性通过将其中两条成功转换成外源染色体片段特异的SCAR标记得到了验证。减法AFLP标记技术的建立为标记作物中的外源染色体片段提供了一种有效手段。
作者 柴建芳 吴志明 赵和 Andre LAROCHE 王海波
机构地区 河北省农林科学院农业物理生理生化研究所 Agriculture and Agri-Food Canada
出处 《Acta Botanica Sinica》 CSCD 2003年第4期379-383,共5页 Acta Botanica Sinica（植物学报：英文版）
关键词 subtracted AFLP subtractive hybridization AFLP alien chromosome fragment WHEAT 减法AFLP 减法杂交 AFLP 外源染色体片段 小麦
分类号 S512.1 [农业科学—作物学]
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参考文献12

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Acta Botanica Sinica
2003年 第4期

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