摘要
马鞍山向山垃圾填埋场建设在向山硫铁矿尾矿库之上、混入玢岩铁矿尾矿和酸性排水,是富含有机质的废物与富含硫酸盐、铁氧化物废物混杂的地带,这是一个微生物电子受体和电子供体长期共存的场所。对这样一个特殊的体系进行微生物矿物交互作用研究具有十分重要的意义。本文对向山垃圾填埋场-尾矿混合带的微生物进行富集培养,经过富集培养和平板划线分离得到一株纯菌。经光学显微和扫描电镜观察、16SrDNA测序、还原硫酸盐能力检测,鉴定该纯菌株为梭状硫酸盐还原菌(Clostridium Sulfate Reducing Bacteria,简称C.SRB)。其生理生化特性研究结果表明,该菌可以利用葡萄糖和乳糖为碳源生长,甲基红试验为阴性,吲哚试验、接触酶试验均为阳性。考查该菌株的生长代谢以及温度、pH、盐度等外界环境影响因素发现,培养该菌株36 h消耗硫酸盐比速率最高为0.73 mmol/g·h;最适生长条件为温度35℃、pH值7、盐度0.5 g/L。
Xiangshan Landfill was constructed above the iron sulfides tailings, mixed with porphyrite iron ore tailings and acidic drainage in Ma'anshan City, China. The landfill is rich in organic waste, sulfates and iron oxide waste. Furthermore, microbial electron acceptor and donor are co-existed there for a long time. Study on microorganism-mineral interaction of this special system is of great importance. Hence, the microorganisms in the mixed area of landfill and tailings in Xiangshan were cultivated. A pure bacteria strain was isolated after enrichment culturing and plate streaking. Optical microscopy, scanning electron microscope, 16SrDNA sequencing and reduction of sulfate ability test were conducted to identify the bacterial strain as Clostridium Sulfate Reducing Bacteria(short for C. SRB). The results of the physiological-biochemical characteristic investigate of this bacteria show that it grows with glucose and lactose as carbon source, methyl red test result shows negative, while both indole test and catalase test results show positive. Besides, the growth and metabolism of the bacteria strain and the external environment factors including temperature, pH, salinity, and etc were studied. It was found that the highest ratio rate for the sulfate consumption is 0.73 mmol·g-1·h-1 when C. SRB was cultivated for 36 h, and the optimum growth conditions are temperature of 35 ℃, pH of 7 and salinity of 0.5 g/L.
出处
《矿物学报》
CSCD
北大核心
2014年第2期175-180,共6页
Acta Mineralogica Sinica
基金
973预研项目(2011CB411904)
国家自然科学基金(批准号:41130206,41372347)
关键词
垃圾填埋场
尾矿
硫酸盐还原菌
生物矿物交互作用
生理生化特性
landfill
tailing
sulfate reducing bacteria
interaction of microbe and minerals
physiological and biochemical characteristics
