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Genetic Diversity of Chinese Medaka(Oryzias sinensis)in Dian Lake Drainage Area by ISSR Analysis 被引量:2

Genetic Diversity of Chinese Medaka(Oryzias sinensis)in Dian Lake Drainage Area by ISSR Analysis
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摘要 Chinese medaka(Oryzias sinensis),widely distributed in the plateau lakes,Yunnan Province,which was the indicative specie for the habitat quality of riparian zone in the wetland ecosystems and also was the key specie in the lake ecosystems,and also was one of the fishes who suffered the severe invasion of alien species.There,we investigated that the genetic diversity and population structure of the wild Chinese medaka by using inter-simple sequence repeat(ISSR)analysis.The samples(20 individuals in total)of Chinese medaka in three populations in the drainage area of Dian Lake were obtained.The 7 ISSR primers were picked out to assess the genetic diversity.The ISSR primers combinations revealed 82.69%polymorphism,among 52 genetic loci amplified.The sum of effective number of alleles and observed number of alleles were 1.492 4 and 1.826 9,respectively.The Shannon genetic diversity and Nei genetic diversity indexes of all the populations were 0.434 5 and 0.289 7,respectively.The gene differentiation(GST)was 0.198 9,and the gene flow(NM)was 2.013 2.The AMOVA demonstrated that only 6.65%of genetic variance came from inter-population,and 93.35%of genetic variance came from intra-population.Phylogenetic tree showed that 20 individuals from 3 populations were clustering mixed each other.Also,the principal coordinate analysis(PCA)supported the cluster analysis results.These results indicated that the genetic diversity of Oryzias sinensis was at the middle level,and there was no obvious genetic differentiation among the three populations of Oryzias sinensis. Chinese medaka(Oryzias sinensis),widely distributed in the plateau lakes,Yunnan Province,which was the indicative specie for the habitat quality of riparian zone in the wetland ecosystems and also was the key specie in the lake ecosystems,and also was one of the fishes who suffered the severe invasion of alien species.There,we investigated that the genetic diversity and population structure of the wild Chinese medaka by using inter-simple sequence repeat(ISSR)analysis.The samples(20 individuals in total)of Chinese medaka in three populations in the drainage area of Dian Lake were obtained.The 7 ISSR primers were picked out to assess the genetic diversity.The ISSR primers combinations revealed 82.69%polymorphism,among 52 genetic loci amplified.The sum of effective number of alleles and observed number of alleles were 1.492 4 and 1.826 9,respectively.The Shannon genetic diversity and Nei genetic diversity indexes of all the populations were 0.434 5 and 0.289 7,respectively.The gene differentiation(GST)was 0.198 9,and the gene flow(NM)was 2.013 2.The AMOVA demonstrated that only 6.65%of genetic variance came from inter-population,and 93.35%of genetic variance came from intra-population.Phylogenetic tree showed that 20 individuals from 3 populations were clustering mixed each other.Also,the principal coordinate analysis(PCA)supported the cluster analysis results.These results indicated that the genetic diversity of Oryzias sinensis was at the middle level,and there was no obvious genetic differentiation among the three populations of Oryzias sinensis.
出处 《湿地科学》 CSCD 北大核心 2015年第5期543-550,共8页 Wetland Science
基金 Under the auspices of Special Program for Key Basic Research Project of the Ministry of Science and Technology,China(973 Program)(2012CB426509) General Project of Research Foundation of Yunnan Provincial Education Department(2014Y335) Research Staring Foundation of Southwest Forestry University(111041)
关键词 ORYZIAS SINENSIS CHINESE MEDAKA genetic diversity Oryzias sinensis Chinese medaka genetic diversity
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  • 1Lifei Zhu,Huili Wang,Huijun Liu,Wei Li.Effect of trifloxystrobin on hatching, survival, and gene expression of endocrine biomarkers in early life stages of medaka ( Oryzias latipes )[J]. Environ. Toxicol. . 2015 (6)
  • 2Bingli Lei,Jia Kang,Yingxin Yu,Jinmiao Zha,Wei Li,Zijian Wang,Yipei Wang,Yu Wen.Long-term exposure investigating the estrogenic potency of estriol in Japanese medaka ( Oryzias latipes )[J]. Comparative Biochemistry and Physiology, Part C . 2014
  • 3Qian Luo,Jin-Miao Zha,Zijian Wang,MingHung Wong,Zongwei Cai.Bioaccumulation and debromination of BDE-209 in Japanese medaka (Oryzias Latipes) when continuously exposed to environmental relevant concentrations[J]. Journal of Environmental Science and Health, Part A . 2013 (11)
  • 4Ge Ding,Daizhen Zhang,Yanqiu Yu,Lingling Zhao,Beibei Zhang.Analysis of genetic variability and population structure of the endemic medicinal Limonium sinense using molecular markers[J]. Gene . 2013 (2)
  • 5Bahman Panahi,Mahmoud Ghorbanzadeh Neghab.Genetic characterization of Iranian safflower ( Carthamus tinctorius ) using inter simple sequence repeats (ISSR) markers[J]. Physiology and Molecular Biology of Plants . 2013 (2)
  • 6Zhenqiao Song,Xingfeng Li,Honggang Wang,Jianhua Wang.Genetic diversity and population structure of Salvia miltiorrhiza Bge in China revealed by ISSR and SRAP[J]. Genetica . 2010 (2)
  • 7Jian-Wen Shao,Wen-Long Chen,Yan-Qiu Peng,Guo-Ping Zhu,Xiao-Ping Zhang.Genetic diversity within and among populations of the endangered and endemic species Primula merrilliana in China[J]. Biochemical Systematics and Ecology . 2009 (6)
  • 8Jian-Yong Liu,Zhao-Rong Lun,Jun-Bin Zhang,Ting-Bao Yang.Population genetic structure of striped mullet, Mugil cephalus , along the coast of China, inferred by AFLP fingerprinting[J]. Biochemical Systematics and Ecology . 2009 (4)
  • 9Yun-Guo Liu,Zhi-Gang Yu,Bao-Long Bao,Xiu-Qin Sun,Qi-Long Shi,Ling-Xiao Liu.Population genetics studies of half-smooth tongue sole Cynoglossus semilaevis using ISSR markers[J]. Biochemical Systematics and Ecology . 2009 (11)
  • 10Marco Casu,Tiziana Lai,Marco Curini-Galletti,Alberto Ruiu,Antonio Pais.Identification of Mediterranean Diplodus spp. and Dentex dentex (Sparidae) by means of DNA Inter-Simple Sequence Repeat (ISSR) markers[J]. Journal of Experimental Marine Biology and Ecology . 2008 (2)

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