摘要
开展了不同外源激素、剂量、注射针距以及环境因子对鳗鲡卵巢发育影响的研究。在所试验的外源激素中,HCG和PT对鳗鲡卵巢催熟效果较好,LHRH-A和TOP则无明显效果。外源激素注射剂量越高,卵巢发育所需时间越短,但注射刘量过低,催熟率则急剧下降。采用注射剂量为0.5mgPT+150IUHCG/500g。在外源激素总剂量不变的情况下,改变注射针距7～15d,除卵巢发育所需时间随针距而延长外,总催熟率不变。超过15d,则催熟率下降。研究认为,鳗鲡卵巢成熟所需外源激素最低总剂量为3.5～4.5mgTP+1050～1350TUHCG/500g。试验的环境因子包括温度、盐度、钙镁比以及pH,除温度高于24℃或低于14℃对卵巢发育有明显的影响外,其它因子在试验范围内对鳗鲡卵巢的发育影响不明显。
The effects of different kinds and dosages of exterior hormones, an interval of time between two injections, as well as environmental factors on the development of Japanese eel ovary were studied. In all kinds of exterior hormones, the effects of HCG and PT on the ovarian development are better, but LHRH-A and TOP are inconspicuous. The higher the dosage, the shorter the period of the ovary for developing maturation. If the dosage is excessively low, the rate of ovarian maturation can be rapidly declined. The optimal dosage adopted at present is 0.5mg PT+150IU HCG. If changing of the interval between injections from 7 to 15 days in the case of fixed total dosage, the total rate of maturation is unchangeable except the time needed for ovarian maturation is changed. But the total rate can fall if the interval exceeds 15 days. The author considers that the inducing ovarian maturation in Japanese eel needed minimum total dosage of exterior hormone should be 3.5～4.5mg PT+1050～1350IU HCG/500g. The environmental factors in the experiment include temperature, salinity, the ratio of calcium to magnesium, to pH. All these values in the experiment do not influence the ovarian maturation except the temperature above 24℃, or under 14℃.
出处
《上海水产大学学报》
CSCD
2003年第1期6-11,共6页
Journal of Shanghai Fisheries University
基金
中国水产科学研究院基金项目资助(2002 1 4)
关键词
外源激素
环境因子
日本鳗鲡
卵巢
exterior hormones
environmental factors
Anguilla japonicus
ovarian maturation
