摘要
从南京地区黄棕壤中分离的一株好氧、革兰氏阴性、产芽孢的硅酸盐细菌NBT菌株 ,能产生丰厚的荚膜 ,具有鞭毛 ,能水解淀粉、产生吲哚、液化明胶 ,全细胞脂肪酸为硬脂酸C16∶0 、软脂酸C18∶1(Δ9) 和anteiso C15,DNA的G +Cmol%为 5 3 7%。 1 6SrRNA基因测序和系统发育学分析的结果表明 ,该菌株与胶质芽孢杆菌B 75 1 9(Bacillusmucilaginosus)、土壤芽孢杆菌B 75 1 7(B .edaphicus)亲缘关系最近。该菌株与B .edaphicusB 75 1 7的总DNA杂交率为 69% ,在形态、生理生化特征上有差异 。
An aerobic gram negative sporulating silicate bacterium is described. Strain NBT was isolated from soil in Nanjing, and has the following characteristics: It fermented a range of sugars and produced acid. Starch hydrolysis, indole formation are positive. The G+C content of DNA was 54 0±0 3% and 16:0 was the major fatty acid. A phylogenetic tree constructed on the 16S rRNA gene sequences clearly indicated that strain NBT formed the same lineage with Bacillus mucilaginosus and B. edaphicus. High level of DNA relatedness (69%) demonstrated the genetic homology between strain NBT and B. edaphicus. Based on the several different phenotypic characteristics, we propose strain NBT as the subspecies of B. edaphicus.
出处
《微生物学报》
CAS
CSCD
北大核心
2003年第2期162-168,共7页
Acta Microbiologica Sinica
基金
上海市科技兴农重点攻关项目资助 (农科攻字 98第 0 5 2号 )
