摘要
目的 :探讨p16及E 钙粘附蛋白 (E cad)基因mRNA在子宫内膜癌中的表达及意义。方法 :采用RT PCR技术检测 3 0例子宫内膜癌及 8例正常子宫内膜组织的p16及E cadmRNA的表达。结果 :①子宫内膜癌p16mRNA的阳性表达明显低于正常子宫内膜 (P <0 .0 1) ,但p16mRNA的表达与子宫内膜癌的组织病理分级无关 ;②E cadmRNA在子宫内膜癌中呈低表达 ,正常组织内呈强阳性表达 ,二者差异非常显著 (P <0 .0 1) ,并且在子宫内膜癌中E cadmRNA的阳性表达与组织病理学分级呈负相关 (P <0 .0 5 )。结论 :p16、E
Objective:To evaluate the significance of the mRNA expression of p16 and E-cad gene in the endometrial carcinoma.Methods:RT-PCR method was used to detect the mRNA expression of p16 and E-cad in 30 specimens of endometrial carcinoma and 8 normal endometrium tissue.Results:①The positive expression of p16 mRNA was significantly lower in endometrial carcinoma specimens than that in normal endometrium tissue(P<0.01),but it’s not related with the pathological grade of endometrial carcinoma.②The mRNA expression of E-cad was lower in endometrial carcinoma but strongly positive in normal endometrium tissue(P<0.01),and there was a negative correlation between the expression of E-cad mRNA and the pathological grade(P<0.05).Conclusions:The mRNA expression of p16 and E-cad genes play an important role in the pathogenesis of the endometrial carcinoma.
出处
《实用妇产科杂志》
CAS
CSCD
北大核心
2003年第2期93-94,共2页
Journal of Practical Obstetrics and Gynecology
基金
黑龙江省自然科学基金资助项目 (编号 D9930 )
