摘要
目的:测定脑缺血低氧后不同时间c-fos和c-jun基因表达。方法:分组:对照组(A);缺氧预处理组(B);缺血低氧组(IH组);B+IH组。采用链霉素亲生物素—过氧化酶免疫组化技术。结果:缺血低氧后30minc-fos和c-jun基因阳性细胞呈低密度分布,在3%～20%之间,1hc-fos基因表达高峰,阳性细胞呈高密度分布,皮层和海马分别为61.3%和56.8%,3hc-fos基因表达下降,12h基本消失。c-jun基因的表达高峰在3h;缺氧适应使缺血低氧脑增加的c-fos基因的阳性细胞数减少,而使缺血低氧脑增加的c-jun基因的阳性细胞进一步增加。结论:缺血低氧可诱发中枢神经系统c-fos、c-jun基因表达,且有时间依赖性;缺氧适应可抑制缺血低氧脑c-fos基因的表达,增强缺血低氧脑c-jun基因的表达。
AIM:To determine the effect of preconditioning on expression of c fos,c jun gene in anoxic ischemic brain.METHODS:Group:Control group,hypoxia preconditioning group,anoxic ischemic,anoxic preconditioning group plus anoxic ischemic group.Expression of c fos,c jun gene in brain were determined with “SP”immunocytohemical assay.RESULTS: The expression of c fos and c jun were not observed in control group. There were scatter expression of c fos and c jun 30 min after anoxic ischemic.Expression of c fos showed the most at 1 h,declined at 3 h,and disappeared at 12 h.But the highest expression of c jun was at 3 h.CONCLUSION: Anoxic ischemic induces central nervous system gene expression of c fos and c jun and time dependent; Hypoxia preconditioning inhibites the gene expression of c fos and increases the gene expression of c jun.
出处
《中国临床康复》
CSCD
2003年第6期931-932,共2页
Chinese Journal of Clinical Rehabilitation
