摘要
目的 对MecA基因探针分析快速检出耐甲氧西林金黄色葡萄球菌 (MRSA)的可靠性和临床实用性进行评估。方法 临床分离的 96株金黄色葡萄球菌中 ,4 4株MRSA采用MecA基因探针快速分析 ,并与MecA基因的PCR结果比较。结果 MecA基因探针快速分析鉴定MRSA的灵敏度为 97.7% ,特异性为 10 0 %。结论 该方法灵敏度高 ,特异性强 ,简单易行 ,90min即可完成 ,是常规实验室检出MRSA快速而可靠的分析方法。
OBJECTIVE Their reliability and clinical practicability of the probe based Velogene rapid MRSA identification assay were evaluated for the ability to identify meticillin resistant Staphylococcus aureus(MRSA). METHODS A total of 96 clinical isolated of S. aureus were tested, consisting of 44 MRSA strains, and 52 meticillin susceptible strains, this assay with PCR for MecA was compared.RESULTS Of the 44 MecA gene positive isolates, 43 were positive by the probe based Velogene rapid MRSA identification assay (sensitivity, 97.7%), and all the MecA gene negative strains were negative by the probe based Velogene rapid MRSA identification assay (specificity, 100%). CONCLUSIONS The assay is a highly sensitive and specific test for detection of meticillin resistance in S. aureus, it offers results within 90 min and is easy to perform, which makes this test a valuable tool in clinical laboratory.
出处
《中华医院感染学杂志》
CAS
CSCD
2003年第2期105-106,共2页
Chinese Journal of Nosocomiology
