摘要
采用胶束电动毛细管色谱法分离测定了中药穿心莲中穿心莲内酯和脱水穿心莲内酯。电泳条件 :以2 0mmol L磷酸缓冲溶液 1 0mmol LSDS(含 5 %甲醇 ,V/V ,pH 6.8)为电泳介质 ,未涂层石英毛细管 (75 μmi.d.× 5 0cm ,有效分离长度 4 2 .2cm)为分离通道 ,压力进样 (2 5 0kPa·s) ,2 0kV恒压电泳 (2 5℃ )分离 ,检测波长 2 4 0nm。在 1 0 .4～93 .5mg/L ,和 1 0 3～ 92 .7mg L范围内 ,对两种内酯分别进行了定量分析。加样回收率穿心莲内酯为 1 0 2 % ,脱水穿心莲内酯为 1 0 2 %。
Andrographic paniculata nees is one of well-known traditional chinese medicinal materials (TCMs), in which andrographolide and dehydroandrographolide are two major active compounds. In this work, a micellar electrokinetic capillary chromatographic (MECC) method has been developed for separation and quantitative analysis of these two compounds in andrographis paniculata ness. Electroretic conditions are as follows: uncoated fused silica capillary (42.2 cm/50 cm (effective/total length), 75 mum i.d.), 20 mmol/L phosphate-10 mmol/L sodium dodecyl sulfate containing 5% methanol (V/V) at pH 6.8, applied voltage 20 W, temperature 25 degreesC, detection wavelength 240 nm, injection 25kPa.s. Both of the analytes were separated successfully within 10 minutes under the optimum conditions. The relative peak areas of the two analytes increase linearly with the increase of their concentrations in the range of 10.4 similar to 93.5 mg/L for andrographolide and 10.3 similar to 92.7 mg/L for dehydroandrographolide, and the revoveries are 102% and 102% respectively.
出处
《分析化学》
SCIE
EI
CAS
CSCD
北大核心
2003年第4期451-454,共4页
Chinese Journal of Analytical Chemistry
基金
国家自然科学基金重点项目 (No .2 0 2 35 0 2 0 )
福建省科技厅中药质量控制重点项目 (No .2 0 0 0F0 0 1)资助课题
