摘要
目的:考察黄连总生物碱的提取测定方法。方法:采用正交实验设计法,以总生物碱得率为考察指标,研究甲醇浓度、加醇量、超声时间3个因素对黄连总生物碱提取的影响。结果:黄连总生物碱的最佳提取方法为加入50倍量的1%盐酸甲醇液,其中甲醇为75%的甲醇,超声提取1h。结论:优选的黄连总生物碱提取测定方法稳定可行。
Objective: To study the method for determination of extraction and total alkaloid in Huanglian. Method: According to the yield of total alkaloid, the orthogonal experimental design was used to study the effect of extract factors on total alkaloid including methanol concentration, the amount of methanol and ultrasonic time. Result: The optimum condition for the total alkaloid extraction was added 50 times 1% hydrochloric acid in 75% methanol solution and ultrasonic extraction 1h. Conclusion: The preferred method of total alkaloid extraction and determination of Huanglian is stable and feasible.
出处
《中药与临床》
2015年第2期28-29,共2页
Pharmacy and Clinics of Chinese Materia Medica
基金
国家中医药管理局中医药行业科研专项(No.201007011)
关键词
黄连
总生物碱
正交设计
Huanglian
total alkaloids
orthogonal design
