摘要
目的:建立银翘解毒丸中多成分含量测定方法,为银翘解毒丸的质量评价与质控标准提供参考依据。方法:采用高效液相色谱法(HPLC),在30℃柱温的条件下,以1 mL/min为流速,岛津C18(4. 6 mm×250 mm,5μm)为色谱柱,使用乙腈-0. 1%甲酸为流动相进行梯度洗脱,分别于327 nm、280 nm与277 nm波长下,测定样品中绿原酸、牛蒡苷与连翘苷的含量。结果:绿原酸在0. 1718~1. 9786μg具有良好的线性范围,平均回收率为99. 47%,RSD值为1. 10%;牛蒡苷在0. 1418~2. 5733μg具有良好的线性范围,平均回收率为99. 32%,RSD值为1. 15%;连翘苷在0. 1573~1. 5787μg具有良好的线性范围,平均回收率为99. 10%,RSD值为1. 32%。四个不同厂家(北京同仁堂、九芝堂、太极集团与药都制药集团有限公司)生产的银翘解毒丸中绿原酸的含量分别为1. 74 mg/g、1. 57 mg/g、1. 18 mg/g、1. 23 mg/g,牛蒡苷的含量分别为2. 48 mg/g、2. 11 mg/g、1. 66 mg/g、1. 75 mg/g,连翘苷的含量分别为0. 32 mg/g、0. 27 mg/g、0. 64 mg/g、0. 51mg/g。结论:高效液相色谱法具有快速简便、准确度高、重复性、专属性与稳定性较好等优点,是一种比较理想的测定银翘解毒丸中各组分含量的方法,可为银翘解毒丸的质量评价提供参考,同时有利于完善质量标准。
Objective:To establish a method for determination of multi-components in Yinqiao Jiedu Pills and provide the reference for the quality evaluation and quality control standards of Yinqiao Jiedu Pills.Methods:Using high performance liquid chromatography(HPLC)at a column temperature of 30℃and a flow rate of 1 mL/min.Shimadzu C18(4.6 mm×250 mm,5μm)was used as a column and using acetonitrile-0.1%formic acid was as a mobile phase for gradient elution.The contents of chlorogenic acid,arctiin and forsythin were determined at 327 nm,280 nm and 277 nm,respectively.Results:Chlorogenic acid had a good linear range of 0.1718-1.9786μg.The average recovery was 99.47%,and the RSD value was 1.10%.The burdock had a good linear range at 0.1418-2.5733μg,and the average recovery was 99.32%.RSD value was 1.15%.The forsythin had a good linear range at 0.1573-1.5787μg and the average recovery was 99.10%,and the RSD value was 1.32%.The content of chlorogenic acid in Yinqiao Jiedu Pills produced by four different manufacturers(Beijing Tongrentang,Jiuzhitang,Taiji Group and Yaodu Pharmaceutical Group Co.,Ltd.)was 1.74 mg/g,1.57 mg/g,1.18 mg/g,1.23 mg/g,respectively.The content of burdock was 2.48 mg/g,2.11 mg/g,1.66 mg/g,1.75 mg/g,and the content of forsythin was 0.32 mg/g,0.27 mg/g,0.64 mg/mg,0.51 mg/g,respectively.Conclusion:High performance liquid chromatography(HPLC)is fast,simple,accurate,reproducible,specific and stable.It is an ideal method for determining the content of each component in Yinqiao Jiedu Pills,providing a reference for the quality evaluation and at the same time help to improve the quality standards.
作者
陈玉璞
CHEN Yupu(Jiaozuo Institute for Food and Drug Control,Jiaozuo 454000,Henan,China)
出处
《辽宁中医杂志》
CAS
2019年第8期1703-1706,共4页
Liaoning Journal of Traditional Chinese Medicine
基金
河南省科技攻关项目(152102110115)
关键词
银翘解毒丸
高效液相色谱
绿原酸
牛蒡苷
连翘苷
含量测定
Yinqiao Jiedu Pills
high performance liquid chromatography
chlorogenic acid
arctiin
forsythin
determination
