摘要
目的:建立黑果小檗根部化学成分的定性鉴别及含量测定方法。方法:采用薄层色谱法考察不同展开条件、展开剂比例、点样量、检视方式、薄层板、展开温度以确定最佳薄层色谱展开系统,并对黑果小檗根部化学成分进行定性鉴别及含量测定。结果:确定氯仿-乙酸乙酯-甲酸(4:4. 5:1. 5),薄层板为GF254硅胶薄层板,点样量为1μL,检测波长为254、366 nm,展开温度为25℃为最佳展开条件,黑果小檗根皮及根木质部中均含有盐酸巴马汀、盐酸小檗碱且盐酸巴马汀、盐酸盐酸小檗碱分别在0. 0211~3. 798、0. 675~4. 05范围内具有较好的线性关系(r=0. 9995、r=0. 9942),其中超声提取的根皮水提物中盐酸巴马汀含量最高,含量为2. 2920 mg/g;超声提取的根皮甲醇提取物中盐酸小檗碱含量最高,含量为3. 7640 mg/g。结论:本实验所建立的薄层展开方法稳定可行,含量测定方法操作简便、重现性好。
Objective:To establish the qualitative identification and content determination methods for chemical composition in Xinjiang Berberis heteropda Schrenk root.Methods:Methods:Thin-layer chromatography was used to determine the optimum thin-layer chromatographic system for different development conditions,the ratio of developer,spotting volume,inspection method,thin-layer plate and deployment temperature,and qualitative identification and content determination methods for chemical composition in Xingjiang Berberis heteropda Schrenk root.Results:Chloroform-ethyl acetate-formic acid(4∶4.5∶1.5)was determined,and the thin layer was a GF254 silica gel thin-layer plate.The sample amount was 1μL.The detection wavelength was254 nm and 366 nm.The development temperature was 25℃.Both the root bark and the root xylem of Xinjiang Berberis heteropda Schrenk contained palmatine hydrochloride,berberine hydrochloride and palmatine hydrochloride and berberine hydrochloride had a good linear relationship in the range of 0.0211-3.798 and 0.675-4.05(r=0.9995,r=0.9942),respectively.Among them,the content of palmatine hydrochloride in the water extract of ultrasonically extracted root bark was the highest and the content was 2.2920 mg/g.The content of berberine hydrochloride in the methanol extract of root barks extracted by ultrasonic was the highest and the content was 3.7640 mg/g.Conclusion:The thin layer development method established in this experiment is stable and feasible.The content determination method is easy to operate and has good reproducibility.
作者
朱慧敏
李莉
李松雅
闫琦
李雯雯
ZHU Huimin;LI Li;LI Songya;YAN Qi;LI Wenwen(College of Pharmacy,Xinjiang Medical University,Urumqi 830000,Xinjiang,China)
出处
《辽宁中医杂志》
CAS
2019年第10期2142-2146,2240,共6页
Liaoning Journal of Traditional Chinese Medicine
基金
国家自然科学基金项目(U1703108)
关键词
黑果小檗根皮
黑果小檗根木质部
薄层色谱
含量测定
Xinjiang Berberis heteropda Schrenk root bark
Xinjiang Berberis heteropda Schrenk root xylem
thin layer chromatography
content determination
