摘要
目的:对补骨脂进行化学成分和抗癌活性研究。方法:采用柱层析对补骨脂氯仿提取物进行分离,通过理化常数和光谱分析鉴定化合物结构,并通过形态学、MTT试验对其抑制人胃癌细胞BGC-823的增殖进行深入研究。结果:从氯仿提取物中分离得到2个化合物,分别鉴定为补骨脂素(psoralea,Ⅰ)和异补骨脂素(IsopsoraleaⅡ);两种化合物对人胃癌细胞BGG-823的半数抑制浓度(IC_(50))分别为5.82μg/ml和148.8μg/ml。结论:化合物Ⅰ,Ⅱ从补骨脂中的分离得率分别为0.48％和0.11％,并对人胃癌细胞BGC-823的生长有较强的抑制作用。
Objective: To study the chemical constituents from Psoralea corylifolia L. and their antitumor activities. Methods:The constituents were isolated on silica gel column chromatography and identified by physicochemical properties and spectroscopic analysis. Morphological and MTT assays in vitro were used to explore the inhibiting effect of the active compounds on the proliferation of BGC-823 cancer cell. Results:Two compounds were isolated from its chloroform extract and identified as psoralen ( I ) and isopsoralen ( II ). IC50 of psoralen and isoporalen were 5. 82 ug/ml and 148. 8 ug/ml respectively. Conclusion:Compound I and II having anti-tumor activity against BGC-823 cancer cell were obtanined from this plant with the yields of 0. 048% and 0. 11%.
出处
《中药材》
CAS
CSCD
北大核心
2003年第3期185-187,共3页
Journal of Chinese Medicinal Materials
