摘要
目的探讨流式细胞术检测多发性骨髓瘤(MM)的免疫表型特征及其临床意义。方法应用多参数流式细胞术(MP-FCM),采用CD138/CD45/SSC设门,检测40例MM患者和20例正常献髓者骨髓细胞免疫表型。结果 MM患者抗原异常表达率由高到低分别为CD45^(dim+/-)(95%)、CD81^(dim+/-)(80%)、CD56^+(70%)、CD27^(dim+/-)(50%)、CD200^(++)(48%)、CD33^+(40%)、CD28^(++)(35%)、CD117^+(18%)、CD19^+(5%)和CD20^+(3%)。CD56+、CD200^(dim+)、CD28^(dim+)在正常浆细胞的表达率分别为10%、10%和5%。结论应用MP-FCM检测MM细胞免疫表型,可准确地鉴别良恶性浆细胞,有助于MM的诊断、预后判断及靶向治疗。
Objective To explore the immunophenotype and its clinical significant of Multiple Myeloma（ MM）. Methods The immunophenotype of 40 patients with MM and 20 normal volunteers were detected by multiparameter flow cytometry（ MP-FCM）. Results The abnormality expression of antigens sequence of patients with MM was CD45 dim+/-（ 95%）,CD81 dim+/-（ 80%）,CD56+（ 70%）,CD27 dim+/-（ 50%）,CD200+ +（ 48%）,CD33+（ 40%）,CD28+ +（ 35%）,CD117+（ 18%）,CD19+（ 5%）,CD20+（ 3%） in order. However the positive rations of CD56+,CD200dim +and CD28dim +were 10%,10% and 5%,respectively. Conclusion MP-FCM inspections can accurately discriminate the benign and malignant plasma cell,and provide evidence for prognosis evaluation and targeted therapy.
出处
《中国临床保健杂志》
CAS
2016年第3期228-231,337,共5页
Chinese Journal of Clinical Healthcare
基金
国家自然科学基金面上项目(81472018)
安徽省自然科学基金面上项目(1408085MH145)
