摘要
目的:检测系统性红斑狼疮(SLE)患者外周血单个核细胞(PBMC)中不同细胞亚群发生凋亡的情况,并初步分析其在SLE发病机制中的意义。方法:取28例SLE患者和性别年龄与之相匹配的20个正常对照者。采用淋巴细胞亚群标志、An-nexin-V及碘化丙啶三色荧光标记、流式细胞术检测细胞的早期凋亡。结果:SLE患者体内CD3+T细胞、CD4+T细胞和CD8+T细胞的凋亡百分率均显著高于正常对照组(P<0.05)。同时,SLE患者T淋巴细胞的死亡百分率也高于正常人。经激素治疗一定时间后,这些细胞亚群的凋亡率会进一步升高(P<0.01),但CD4/CD8比值恢复至接近正常人。SLE患者体内CD19+B淋巴细胞的凋亡或死亡百分率和正常对照组相比均无明显差别,激素治疗对B细胞凋亡和死亡的影响亦不显著。结论:在SLE患者体内主要是T淋巴细胞凋亡增加,但SLE患者淋巴细胞数目减少不仅仅是因为细胞凋亡所致,死亡细胞数目明显升高也是其中一个重要原因。激素治疗可诱导SLE患者T淋巴细胞进一步发生凋亡,并使T细胞亚群比例有所改善。
Objective: To investigate the abnormal apoptosis of the subpopulation of peripheral blood mononuclear cells(PBMC) from SLE patients and to evaluate the possible pathological contribution of PBMC apoptosis to SLE disease. Methods: Three-colour immunofluorescence and flow cytometry were employed to determine the percentages of early apoptic subsets by binding Annexin V-FTTC but not PI and of dead cells by binding both Annexin V-FITC and PI. Results: Compared with normal controls, there were significantly higher apoptic and dead cells in CD3+T cells,CD4+T cells and CD8+T cells but not in CD19+B cells from SLE patients. After glucocorticoid (GC) treatment, the percentages of apoptosis in CD3+T cells, CD4+T cells and CD8+T cells further increased significantly in SLE patients while the ratio of CD4/CD8 recovered approximately to normal level. Conclusion: The percentage of early apoptic T lymphocytes is significantly higher than normal controls and will be much more increased by GC treatment. It is possible that the increased apoptosis of T lymphocytes in SLE is only a result of abnormal activation and GC treatment may induce the lymphocytes to apoptosis and normalize the ratio of to CD4+/CD8+, which may alleviate the patients' severe symptoms.
出处
《南京医科大学学报（自然科学版）》
CAS
CSCD
北大核心
2003年第3期231-234,共4页
Journal of Nanjing Medical University(Natural Sciences)
基金
江苏省自然科学基金资助项目(BK99135)
关键词
红斑狼疮
系统性
细胞凋亡
三色荧光标记
流式细胞术
lupus eiythematosus, systemic
apoptosis
three-colour immunofiuorescence
flow cytometry
