摘要
目的 建立兔血浆中胡芦巴碱的高效液相色谱测定法 ,并研究其在兔体内的药代动力学过程。方法 分别给兔ig胡芦巴提取物 ,及iv胡芦巴碱单体 ,不同采血点的血浆样品经甲醇、乙腈沉淀蛋白后 ,以乙腈 水 (90∶10 )为流动相 ,采用AsahipakNH2 P 5 0色谱柱分离 ,流速 1 2mL·min- 1 ,检测波长 2 65nm ,柱温为 3 0℃。结果 胡芦巴碱线性范围为 0 98～ 3 1 2 8mg·L- 1 ,血浆检测限为 5 0 μg·L- 1 。兔ig胡芦巴提取物及iv胡芦巴碱生理盐水溶液后 ,其药时过程分别符合一室和二室开放模型特征。结论 胡芦巴碱在兔体内是一种中速吸收 ,快速消除的药物。该测定方法操作简便、快速、准确 。
Aim To develop a sensitive and specific HPLC method for determination of trigonelline in rabbit plasma, and study the pharmacokinetics in rabbit. Methods After ig of fenugreek extract and iv of trigonelline in rabbit, the biological samples could be well purified after precipitation of protein with methanol and acetonitrile. Asahipak NH 2P 50 column was used,the mobile phase consisted of acetonitrile water (90∶10) at a flow rate of 1 2 mL·min -1 , and detection wavelength was set at UV 265 nm. The column temperature is 30 ℃ . Results The calibration curve was linear in the range from 0 98 mg·L -1 to 31 28 mg·L -1 , with r =0 9986 , the detection limit of this method was 50 μg·L -1 . The concentration time curves of trigonelline in rabbits after ig and iv administration were shown to fit one compartment and two compartment open model, respectively. The main parameters after ig of fenugreek extract were as follows: T 1/2( K a) was 0 9 h, T 1/2( K e) was 2 2 h, V was 0 64 L·kg -1 , AUC was 1 93 mg·min·L -1 . The main parameters after iv of trigonelline were as follows: T 1/2 α was 10 8 min, T 1/2 β was 44 0 min, K 21 was 0 044 min -1 , K 10 was 0 026 min -1 , K 12 was 0 017 min -1 , AUC was 931 0 mg·min·L -1 . Conclusion Trigonelline showed a middle rate of absorption and fast rate of elimination in rabbit. Meanwhile, the method is simple,accurate,with a good reproducibility, and it provide a basic method for the investigation of trigonelline and fenugreek pharmacokinetics.
出处
《药学学报》
CAS
CSCD
北大核心
2003年第4期279-282,共4页
Acta Pharmaceutica Sinica
