摘要
为探讨环氧合酶 2 (COX 2 )在大鼠酒精性肝损伤中的作用 ,将大鼠随机分为 3组。正常组 :予以玉米油 +葡萄糖液灌胃 ;模型组 :玉米油 +酒精灌胃 ;处理组 :玉米油 +酒精 +塞莱昔布 (celecoxib,为COX 2选择性抑制剂)灌胃。 3周后处死大鼠 ,然后进行大鼠肝组织切片HE染色、脂肪染色及COX 2免疫组化染色 ;血清AST、ALT水平、血浆及肝组织匀浆谷胱甘肽 S转移酶 (GST)活性测定。结果发现 ,模型组脂肪染色明显加重 ,AST升高 ,肝GST活性降低 ,COX 2表达增加 ;而处理组比模型组脂肪染色减轻 ,AST下降 ,GST活性上升 ,肝组织中COX 2表达明显减少。提示使用选择性COX 2抑制剂能减轻大鼠酒精性肝损伤 ,证实COX 2参与了大鼠酒精性肝损伤过程。
The effects of cyclooxygenase 2 (COX 2) on alcohol induced liver injury in rats were investigated. Rats were randomly divided into three groups: Control group was given with corn oil plus dextrose; Model group with corn oil plus ethanol; Treatment group with corn oil plus ethanol and a selective COX 2 inhibitor celecoxib. All materials were injected into stomach through intragastric tubes. Liver samples were analyzed with histopathology, fatty staining and immunohistochemistry. Glutathione s transferase (GST) activity of liver tissue and plasma were detected. Levels of aspartate aminotransferase (AST) and alanine aminotransferase (ALT) in serum were measured. Compared with the control group, positive fatty staining cells and expression of COX 2 in liver, level of ALT and AST in serum and activity of GST in plasma were all significantly increased in the model group, but the GST activity in liver was decreased. However, these changes were all reversed in the treatment group. The results show that the model of alcohol induced liver injury in rats has been set up successfully, the selective COX 2 inhibitor celecoxib can protect against alcohol induced liver injury in rats and COX 2 may play a role in alcohol induced liver injury
出处
《解放军医学杂志》
CAS
CSCD
北大核心
2003年第4期340-342,F003,共4页
Medical Journal of Chinese People's Liberation Army
关键词
环氧合酶-2
大鼠
酒精性肝损伤
作用
免疫组化
cyclooxygenase 2
alcoholic liver injury
selective COX 2 inhibitor
glutathione s transferase
