摘要
研究了酸性染料氯酚红与蛋白质的结合反应。在pH为4.00的邻苯二甲酸氢钾缓冲介质中,氯酚红与蛋白质通过分子间作用力形成复合物。使最大波长约为340nm的共振光散射光谱得到加强。基于这一现象可以测定低至0.020mg·L^(-1)的血清白蛋白,工作曲线在0～0.75mg·L^(-1)范围内呈线性关系。此方法的稳定性好,灵敏度高,用于人血清试样中总蛋白的测定,与常用的双缩脲法基本一致,且灵敏度高。
A new resonance light scattering (RLS) assay is presented in this paper. At the optimum pH = 4.0, the weak RLS of chlorophenol red can be greatly enhanced by the addition of proteins due to the interaction between protein and chlorophenol red. A new quantitative determination method for proteins has been developed. The linear range is 0-1.0 mg . L-1 for BSA, and 0-0.75 mg . L-1 for HSA with detection limit of 20.3 mug . L-1 for BSA, and 20.4 mug . L-1 for HSA, respectively. Besides high sensitivity, the method is characterized by good reproducibility, rapidity of reaction, good satability and few inferring substances. The determination results of the proteins in human serum samples by this method are very close to those obtained using biuret spectrophotometric method, with relative stand deviation of 1.17%-2.42%.
出处
《光谱学与光谱分析》
SCIE
EI
CAS
CSCD
北大核心
2003年第2期229-231,共3页
Spectroscopy and Spectral Analysis
基金
河北省教委博士基金
关键词
氯酚红
共振光散射法
定量测定
蛋白质
人血清
临床分析
protein
resonance light scattering
chlorophenol red
human serum albumin
bovine serum albumin
