摘要
目的 探讨髓过氧化物酶 (MPO)基因多态性与苯致血液毒性易感性的关联。方法 采用多聚酶链反应 -限制片段长度多态性 (PCR RFLP)技术和DNA测序法 ,分析 35例慢性苯中毒患者、46名苯作业工人和 2 6名健康工人的MPO基因转录起始位点上游 463位点等位基因分布情况 ,比较各组间的差别 ,探讨MPO基因多态性与苯中毒危险性的可能关联。结果 MPO基因上游 463位点存在G/G、G/A、A/A 3种基因型 ;G/G基因型在苯中毒病例组分布频率较苯接触组高 2 7.1 4 % ;MPO基因 463位点具G/G基因型个体发生苯中毒的危险性比G/A +A/A基因型增加 2 .8倍 (OR =2 .835 ,95 %CI:1 .0 65～ 7.549,P <0 .0 5) ;MPO基因多态性与苯中毒关联不存在性别差异。结论 在相同的苯作业暴露环境中 。
Objective To study the relationship between the polymorphism myeloperoxidase(MPO) gene and the genetic susceptibility to benzene toxicity in workers exposed to benzene and in patients with benzene poisoning. Methods Using polymerase chain reaction restriction fragment length polymorphisms(PCR RFLP) techniques,the genotypes' polymorphism of MPO gene in 35 patients with chronic benzene poisoning,46 workers exposed to benzene from the same workplace(as exposed control) and 26 controls were analyzed. Result There were three(G/G,G/A and A/A) genotypes in the region of 463 bp upstream of MPO gene.The distribution frequency in G/G wild type genotype in patients was 27.4% more than that in the exposed workers.The risk of benzene hematotoxicity in those with G/G genotype was 2.8 fold higher than G/A+A/A genotype( OR =2.835,95% CI :1.065～7.549, P <0.05).The polymorphism of myeloperoxidase was not associated with gender specific. Conclusion In the same benzene exposed environment,the subjects with MPO 463 G/G genotype may be more susceptible to benzene toxicity.
出处
《中华劳动卫生职业病杂志》
CAS
CSCD
北大核心
2003年第2期86-89,共4页
Chinese Journal of Industrial Hygiene and Occupational Diseases
基金
国家自然科学基金资助项目(39870 6 85)
