摘要
目的 探讨糖尿病 (DM)状态下葡萄糖 (Glu)对人脐静脉内皮细胞 (HUVEC)凋亡的影响及其分子机制。 方法 细胞凋亡用吖啶橙 (AO)荧光染色、原位末端标记 (TUNEL)法、流式细胞仪分析检测。同时行bcl 2和bax免疫细胞化学染色和定量分析。 结果 高浓度Glu( 2 0mmol/L、4 0mmol/L)能够诱导HUVEC凋亡 ,且呈浓度和时间依赖性。高糖 ( 2 0mmol/L)培养HUVEC 72h后bax表达的MOD×area值由 387± 97升至 136 9± 2 2 5 (P <0 .0 1) ,对照组和高糖组bcl 2染色的MOD×area分别为 15 0± 36和 186± 76 ,两者间差异无显著意义 (P >0 .0 5 )。 结论 DM状态下高血糖能诱导内皮细胞凋亡 ,其机制可能与bax基因表达上调有关。
Objective To investigate the influence of high glucose on the induction of apoptosis of human umbilical vein endothelial cells (HUVEC) in vitro and to explore its potential molecular mechanismes. Methods Apoptosis of HUVEC were observed by acridine orange (AO) fluorescence staining and TUNEL assay, and quantitated by flow cytometry using propidium iodid(PI) staining. The expression of bcl 2 and bax protein were evaluated by immunohistochemical staining with anti human bcl 2 and bax antibodies. Results Highglucose (20 mmol/L、 40 mmol/L) may induce HUVEC apoptosis.Apoptosis of HUVEC was positively corrlated with concentrations of glucose and time dependent. Incubation of HUVEC with high glucose for 72 h increased the values of MOD×area of bax (1369±225) versus incubation with low glucose (387±97, P<0.01 ), and the values of bcl 2 were unchanged (150±36 versus 186±76, P>0.05 ). Conclusion Highglucose may induce HUVEC apoptosis in vitro by up regulated expression of bax.
出处
《中国糖尿病杂志》
CAS
CSCD
2003年第1期37-41,共5页
Chinese Journal of Diabetes
基金
教委科研基金项目资助
