摘要
目的 :研究正常生育大鼠和腺嘌呤诱导的不育大鼠附睾精子中精蛋白及其 m RNA的含量以及它们的相关性。方法 :大鼠喂服腺嘌呤制备不育动物模型 ,收集对照组和不育组大鼠附睾精子 ,提取总碱性核蛋白 ( TNBP) ,电泳分析 ;提取总 RNA,用 RT- PCR分析精蛋白 m RNA相对含量。结果 :不育组大鼠附睾精子中精蛋白含量明显低于正常组 ( P<0 .0 1 ) ,对精蛋白 m RNA的分析也得到了相似结果 ,正常组和不育组相比较 ,有非常显著差异 ( P<0 .0 0 1 )。结论 :精子中精蛋白 m RNA的相对含量能反映出精子中精蛋白的相对含量 。
Objective: To investigate the protamine and protamine mRNA in epididymal sperms of normal and infertile rats.Methods: Infertile rats were induced by adenine treatment. Total nuclear basic protein (TNBP) of epididymal sperms was extracted and analysed by gel electrophoresis. Total RNA was isolated and relative amount of protamne mRNA was studied by means of the RT PCR techniques. Results: The content of protamine in epididymal sperms of infertile rats was much less than normal rats. Same results were obtained by analysis of protamine mRNA. Conclusion: The relative amount of protamine mRNA in sperms could represent the content of protamine in sperms. These results may became valuable for new genetic diagnosis for male infertility.
出处
《生殖与避孕》
CAS
CSCD
北大核心
2003年第2期65-68,共4页
Reproduction and Contraception
基金
国家重点基础研究基金 ( G19990 5 390 1)资助项目
