摘要
目的 建立宫颈上皮肉瘤样病变(Cervical intraepithelial neoplasia,CIN)和各期宫颈癌组织端粒酶含量的测定方法,研究其在宫颈癌早期诊断中的价值。方法 以正常宫颈组织为对照,收集病理证实为宫颈内瘤样变和各期宫颈癌组织标本,获取宫颈组织细胞,采用异硫氰酸荧光素(Fluoresceinisothiocyanate,FITC)间接荧光标记法对端粒酶进行标记后,流式细胞仪进行定量检测。以端粒酶表达率及端粒酶表达的荧光指数为指标进行统计学分析。结果 ①端粒酶表达的百分率及荧光指数两指标在宫颈癌、CIN及正常对照的组间差异显著,且有渐减规律;②宫颈癌不同期别的组间差异显著。结论 端粒酶含量在癌前病变中已表现出显著升高,且其含量随肿瘤的发展而进行性升高,因此端粒酶的定量检测对宫颈癌的早期诊断及检测肿瘤浸润具有重要的临床价值。
Objective To establish the detection method of telomerase level in cervical intraepithelial neoplasia(CIN) tissue and cervical carcinoma tissue, and study its clinical significance. Methods The tissue cells from the cases with CIN and cervical carcinoma and normal cervix were collected, stained by fluorescein isothiocyanate(FITC) and detected by flow cytometry(FCM).Then, statistics analysis of the telomerase quantity and fluorescence index was performed. Results (1)The statistical index had significant difference among CIN group, cervical carcinoma group and control group. The statistical index were increasing along with the advancing of histologic differentiated stages in cervical carcinoma. (2)The statistical index of the different stage in cervical cancer had significant difference in the three groups. Conclusion Telomerase content increase in precancerous lesions and early cervical carcinoma and invasion cervical carcinoma, thus it is helpful for early diagnosis of cervical carcinoma and evaluation of invasion cervical carcinoma.
出处
《实用医药杂志》
2003年第4期286-287,共2页
Practical Journal of Medicine & Pharmacy
关键词
宫颈上皮内瘤样病变
宫颈癌
端粒酶
Cervical intraepithelial neoplasia Cervical cancer Telomerase
