摘要
报道了从云南红豆杉 (TaxusYunnanensis)树皮和枝条中分离得到的 5 2株内生真菌中 ,有 19株菌的发酵产物经TLC分析和HPLC检测验证后产紫杉醇或紫杉烷类物质。其中紫杉醇含量在 1 以上的菌株有 8株。TAX - 4 7(茎点霉属 )和TAX - 4 9(组丝核菌属 )的紫杉醇得率分别为 4 7 30 2 μg L和 31 0 6 μg L ,TAX - 2 5 (茎叶核菌属 )巴可亭Ⅲ得率为 74 4 2 μg L 。
strains of endophytic fungi are isolated from inner bark and branches of Taxus yunnanensis With the analysis of TLC and test of HPLC,of 52 strains of endophtic fungi,19 strains can produce taxol and Taxane,among which the content of 8 strains of taxol is more than 1 The taxol yield of TAX-47(phoma) and TAX-49(phacodium) are 47 302μg/L and 31 06μg/L, respectively TAX-25(Ectostroma) can produce baccatin Ⅲ with the yield of 774 2μg/L These endophytic fungi are worth making a further study
出处
《生物技术》
CAS
CSCD
2003年第2期10-11,共2页
Biotechnology
