摘要
以麻疯树(Jatropha curcas)的种子、叶柄、叶片为实验材料进行愈伤组织的诱导及快速繁殖的实验.用不同浓度的BA和IBA对其不同外植体进行试验,发现用MS培养基加0.5 mg/L BA和1 mg/L IBA对叶片的效果最佳.在相同BA浓度处理条件下,减小IBA浓度会对下胚轴愈伤组织的出芽产生明显的效果.叶柄要求的浓度更低,0.1 mg/LBA和0.1 mg/L IBA为最佳.不定芽在无激素的MS培养基中进行生根培养,通过几天的练苗过程,就能转到土壤中生长.
The explants of Jatropha curcas, hypocotyl, blade and petiole, were used for the test on MS with BA and IBA. The induction of shoot bud was recorded highest on MS medium with 0. 5 mg/L BA and 1 mg/L IBA from blade. Under the condition with the same BA concentration, if IBA concentration was reduced, it would be obviously effective for budding from hypocotyl callus. The petioles required much lower concentrations of the two growth regulators (0. 1 mg/L BA + 0. 1 mg/L IBA) . Regenerated shoots were rooted on growth regulator-free MS medium and could be transplanted in soil after simply hardening for several days. Fig 1 , Tab 2, Ref 13
出处
《应用与环境生物学报》
CAS
CSCD
2003年第2期127-130,共4页
Chinese Journal of Applied and Environmental Biology
基金
国家科技攻关计划课题(编号2002BA901A15)资助~~
