摘要
目的检测尿路致病性大肠杆菌 (uropathogenicEscherichiacoli,UPEC)临床株I型菌毛的携带频率 ,并对其粘附素基因FimH作序列分析。方法用血凝方法检测尿路致病性大肠杆菌的I型菌毛 ;根据GENEBANK的大肠杆菌I型菌毛粘附素FimH基因序列设计引物 ,用聚合酶链反应 (polymerasechainreaction ,PCR)检测临床分离的 15 5株UPEC的目的基因 ,选取典型阳性株的扩增产物测序。结果 15 5株尿路致病性大肠杆菌 ,用血凝方法仅检测到 5 5株携有I型菌毛 ,阳性率 3 5 .5 % ;而用基因扩增方法检测 14 0株 (90 .5 % )含有FimH基因。扩增产物序列与尿路致病性大肠杆菌标准株J96的序列基本一致。结论FimH基因存在于大多数尿路致病性大肠杆菌临床株中 ,是其重要毒力因子 ,具有重要的临床意义。
ObjectiveTo detect the frequency of I type fimbria and analyse the sequence of FimH gene of uropathogenic Escherichia coli(UPEC) clinical isolates.MethodsI type fimbria was detected by hemoglutination.The primers were designed according to the DNA sequence of FimH of I type fimbria in UPEC. The FimH gene of 155 clinical isolates of UPEC was detected by polymerase chain reaction(PCR), and a classical positive PCR product was sequenced. ResultsAmong 155 isolates, I type fimbria was detected in 55 strains( 35.5 % ) by hemoglutination, but 140( 90.5 % ) strains were found to have FimH gene by PCR, the sequence of PCR products was the same as J96. ConclusionFimH gene is present in most of UPEC clinical isolates, as important virulent factor and has clinical significance.
出处
《河北医科大学学报》
CAS
2003年第3期136-138,共3页
Journal of Hebei Medical University
