摘要
目的 :研究HBVDNA含量与乙型肝炎血清学免疫标志物表达关系及HBVDNA含量与肝细胞损害关系。方法 :以完全闭管式的PCR和荧光探针技术相结合的实时检测定量PCR方法 ,对 110例病毒性肝炎患者血清HBVDNA进行检测。结果 :HBeAg阳性 /抗HBe阴性患者HBVDNA拷贝数与HBeAg阴性 /抗HBe阳性患者或HBeAg阴性 /抗HBe阴性患者的拷贝数比较差异有显著性 (P <0 .0 5 ) ,而后二者比较差异无显著性 (P >0 .0 5 ) ;HBVDNA拷贝数与肝实质损害指标血清谷丙转氨酶 /谷草转氨酶 (ALT/AST)、总胆红素 (TBIL)、白蛋白 (ALB)、凝血酶原活动度 (PTA)不具相关性。结论 :抗e抗原抗体非病毒复制终止的标志、病毒性肝炎的肝实质损害程度与血清HBVDNA的含量无相关性。
Objective To study the relationship between the serum contents of HBV DNA copies and the HBV serum index of immunology and the degree of hepatocyte injury in patients with HBV hepatitis. Methods The contents of HBV DNA copies were detected by fluorescence quantitative PCR(FQ-PCR),and the HBV serum index of immunology by ELISA. Results The contents of HBV DNA copies had a significant difference between HBeAg (+)/anti-HBe (-) group (42 patients)and HBeAg (-)/anti-HBe (+) group (57 patients)or HBeAg (-)/anti-HBe (-) group(11 patients)(P<0.05),while there was no significant difference between HBeAg (-)/anti-HBe (+) group and HBeAg (-)/anti-HBe (-) group(P>0.05). The contents of HBV DNA copies had no correlation with the severe degree of hepatocyte injury(P>0.05).Conclusion Anti-HBe is not the terminal sign of HBV replication .The severe degree of hepatocyte injury has not a direct correlation with the contents of HBV DNA copies.
出处
《湖南医科大学学报》
CSCD
北大核心
2003年第2期127-128,共2页
Bulletin of Hunan Medical University
