摘要
目的 研究细胞内Ca2 + 浓度 [Ca2 + ]i的升高对人乳腺癌MCF 7细胞株中Caspase 3 ,Bax及Bcl 2表达的影响。方法 在体外培养下 ,经不同浓度thapsigargin(TG )处理的MCF 7细胞株用荧光指示剂Fura 2 /AM于单波长荧光分光光度仪上测定细胞内钙离子浓度。用免疫组织化学方法检测Caspase 3 ,Bax及Bcl 2的表达。结果 随着细胞内钙离子浓度的提高 ,Bax表达逐渐增强 ,Bcl 2表达逐渐减弱 ,而Caspase 3始终呈阴性表达。 结论 细胞内钙离子浓度的升高对MCF 7细胞株可诱发不通过Caspase 3途径的细胞凋亡。
Objective To study the effect of rising [Ca 2+ ]i on the expression of Caspase 3, Bax and Bcl 2 in human breast cancer MCF 7 cell lines. Methods After the MCF 7 cells treated with different dose of thapsigargin(TG), intracellular calcium concentrations in MCF 7 cells were measured by calcium sensitive fluorescence indicator Fura 2/AM technique; and immunohistochemical method was used to detected the expressions of Caspase 3, Bax and Bcl 2. Results Bax was over expressed and Bcl 2 was down expressed in a [Ca 2+ ]i concentration dependent way;nevertheless, the expression of Caspase 3 was negative in all the TG treated cells. Conclusions The increase of intracellular calcium concentration may have an inductive effect of apoptosis on MCF 7 cells, which is independent to Caspase 3.
出处
《中国普通外科杂志》
CAS
CSCD
2003年第5期354-356,共3页
China Journal of General Surgery
