摘要
以优良樱桃砧木新品系 98 1、Colt、大青叶为试材进行叶片离体再生及根癌农杆菌介导遗传转化 ,建立了高频率再生系统 ,并获得抗菌肽转基因植株。诱导叶片再生的最佳培养基为MS附加BA 1.0～2 .0mg/L、NAA 0 .3～ 0 .5mg/L、GA 0 .5mg/L、AgNO3 5 .0～ 10 .0mg/L。农杆菌及受体感受态是影响转化的关键 ,延迟筛选可提高叶片中转化细胞对卡那霉素的抗性而利于再生。PCR及SouthernBlot检测为阳性 ,表明抗菌肽基因已整合到樱桃砧木 98 1基因组。
The leaf regeneration and Agrobacterium tumefaciens-mediated transformation system of cherry rootstock 98-1,Colt and Dagingye were established at the first time.The results showed that the best medium was Ms supplemented with 1.0-2.0mg/L BA,0.3-0.5mg/L NAA,0.5mg/L GA and 5.0-10.0mg/L AgNO 3 promoting leaf regeneration frequency.The physiological reaction of Agrobacterium tumefaciens and explant cell were key to transformation.Delay selection could improve resistance to Kan of transformed cell in leaves and do well to regeneration.The results of molecular biological assays with PCR and southern blot hybridization preliminarily indicated that the antibacterial peptide genes had been integrated into the genome of cherry.
出处
《园艺学报》
CAS
CSCD
北大核心
2003年第2期209-211,共3页
Acta Horticulturae Sinica
基金
辽宁省攻关项目 (992 0 80 0 1)
关键词
樱桃
砧木
叶片再生系统
抗菌肽
基因转化
Cherry
Leaf
Regeneration
Antibacterial peptide gene
Agrobacterium tumefaciens-mediated transformation
