摘要
目的优选蒙药耧斗菜中总黄酮的提取条件,测定三批次蒙药耧斗菜总黄酮的含量。方法采用正交试验法,以乙醇为提取溶剂,通过对提取时间、料液比、乙醇浓度三个影响因素对蒙药耧斗菜中总黄酮的提取条件进行优化,采用紫外分光光度法测定总黄酮含量。结果通过正交试验方法得出提取总黄酮的最佳回流条件为:浓度为70%的乙醇,料液比为1:20,提取2h;以芦丁为对照品,得出回归方程A=10.665 0C+0.007 85,r=0.999 5,线性范围为14.08~84.48μg/m L;平均加样回收率为98.82%(n=4);6月初、末采于大青山小井沟与呼市赛罕区的蒙药耧斗菜总黄酮的含量分别为0.919mg/g、1.339mg/g及1.316mg/g。结论本法科学,操作简便,经济,适用于蒙药耧斗菜中总黄酮的有效提取与含量测定。
objective the paper is optimizing the extraction conditions of reflux of total flavonoids in Aquilegia viridiflora Pall.,and determinating its contents according to the conditions. Methods with the method of orthogonal test,using ethanol as extraction solvent,this paper optimizes the extraction condition from three factors,the extraction time,ratio of material- liquid,and the concentration of ethanol. UV- Vis Spectrophotometry is used to determinate the content of total flaconoids in Aquilegia viridiflora Pall.. Results By using the orthogonal experiment method,the optimum reflux extraction conditions of flavonoids were found as follows: the concentration of ethanol was 70%,the ratio of material- liquid( g: m L) was 1: 20,the time of extraction was 2h. By using rutin as comparison,the regression equation was obtained as follows,A = 10. 665 0C + 0. 007 85,r = 0. 999 5,the linear range was 14. 08 ~ 84. 48 ug / m L and the average recovery was 98. 82%( n = 4). The content of total flavonoids in Aquilegia viridiflora Pall. was 0. 919 mg / g,1. 339 mg / g and 1. 316 mg / g,which were collected from the Mount Daqing in Xiaojinggou and Saihan District of Hohhot in the beginning and end of June. Conclusion The method usd in this paper is scientific,operational and economy,which is suitable for the effective extraction and content determination of total flavonoids in Aquilegia viridiflora Pall.
出处
《内蒙古农业大学学报（自然科学版）》
CAS
2015年第1期1-5,共5页
Journal of Inner Mongolia Agricultural University(Natural Science Edition)
基金
内蒙古自然科学基金(2013MS1222)
关键词
耧斗菜
总黄酮
正交试验
含量
Aquilegia viridiflora pall
total flavonoids
orthogonal test
content
