摘要
建立了分析土壤和水中的莠去津农药残留的反相高效液相色谱方法。使用去离子水对土壤中的莠去津进行提取 ,Sep PakC1 8反相固相萃取柱对土壤浸提液和水样进行富集、浓缩、纯化 ,并用 1 .5～ 2mL的甲醇洗脱 ,利用甲醇 -水作为流动相 ,采用等梯度淋洗技术进行高效液相色谱分析。莠去津在土壤中不同添加水平回收率分别为 87.0 %～ 93.3%,水中则为 97.3%～1 0 3.2 %,介于 80 %～ 1 2 0 %。莠去津的最小检出量为 0 .0 1ng ,土壤和水中莠去津最低检出浓度分别为 1 .5ng/ g和 0 .0 3μg/L水平。该方法由于用水提取及固相萃取技术的使用 ,使整个前处理过程有机试剂的使用量仅为数毫升 。
An improved HPLC method was developed for the determination of atrazine in soil and water samples. The method involves an initial extraction of the soil samples with water. Sep pak C 18 SPE cartridges were used to concentrate atrazine from both soil extraction and water samples and then eluted by air displacement of 1.5～2 mL of methanol. Atrazine was analyzed by a reversed phase high performance liquid chromatography using a column of Nova Pak C 18 bonded silica with methanol water as a mobile phase. Detection wavelength was 222 nm. The average recoveries of spiked analytes were fall within 87.0%～93.0% and 97.3%～103.2% for soil and water samples respectively.The detection limit is 0.01 ng for atrazine. The detection level of atrazine is 1.5 ng/g in soil and 0.03 μg/L in water. The method has been applied to the determination of atrazine residue in soil and water samples. And it can be used in monitoring atrazine pollution in environment.
出处
《岩矿测试》
CAS
CSCD
北大核心
2003年第2期93-97,共5页
Rock and Mineral Analysis
基金
国土资源部科技项目 (2 0 0 1 0 30 1 )
