摘要
目的:了解红豆杉细胞提取物对肝癌细胞系SMMC-7721生长的影响。方法:运用MTT比色法测定癌细胞的存活率和流式细胞仪分析细胞周期。结果:红豆杉细胞二氯甲烷提取物对癌细胞SMMC-7721的抑制作用远大于甲醇提取物,IC_(50)值分别为0.0834 mgDCW.ml^(-1)和0.8002 mg DCW.ml^(-1);流式细胞仪分析发现外加红豆杉细胞二氯甲烷提取物后肝癌细胞G_2/M期含量增加。结论:红豆杉细胞提取物在体外对肝癌细胞SMMC-7721的生长通过诱导凋亡而具有抑制作用。
Objective:To analyze the cytotoxicity and inhibitory effect of extracts from cultured cells (from stems) of Taxus chinensis on cancer cell line SMMC-7721. Methods:MTT assay for cell viability and flow cytometry for cell cycle analysis. Results:MTT results showed that the extact by dichloromethane was more effective than that by methanol on SMMC-7721, IC50 were 0. 0834 mgDCW. ml-1 and 0. 8002 mg DCW. ml-1 respectively. SMMC-7721 cells in G2-M stage increased with higher concentration of dichloromethane extracts from Taxus chinensis cells and longer incubation. Conclusion:Extracts from cultured cells of Taxus chinensis could have cytotoxic effect on SMMC-7721 and could induce apoptosis of cancer cells.
出处
《中药材》
CAS
CSCD
北大核心
2003年第5期335-337,共3页
Journal of Chinese Medicinal Materials
基金
国家"九五"资助项目(96-C02-03-01)
国家863资助项目(102-12-06-01)
