摘要
目的:研究藤茶总黄酮(Tengcha flavonoids,TCF)清除氧自由基与抗脂质过氧化作用。方法:以黄嘌呤-黄嘌呤氧化酶系统产生的超氧阴离子自由基,研究TCF对氧自由基的清除作用;以Vc-Fe^(2+)系统诱导大鼠肝匀浆及肝线粒体产生的脂质过氧产物,研究TCF抗脂质过氧化作用。结果:TCF能清除氧自由基,其IC_(50)为14.7mg/L;能抑制大鼠肝匀浆自氧化及和Vc-Fe^(2+)系统诱导引起的脂质过氧化,对肝线粒体也有保护作用,并呈浓度依赖关系。结论:TCF对氧自由基有清除作用,并能预防性对抗超氧阴离子自由基引起的氧化性损伤。
Objective:To study the effects of Tengcha flavonoids (TCF) on scavenging oxygen free radicals and inhibiting lipid-peroxidation. Methods: The effects of TCF on scavenging superoxide anion radical induced by xanthine-xanthine oxidase, inhibiting lip-id-peroxidation of heart, liver, brain tissue homogenate and mitochondria induced by Fe2+-VitC, Fe 2+-H2O2, Fe-Cys were discussed by TBA spectrometric method. Results:TCF could significantly inhibit the superoxide anion radical, IC50 is 14. 7 mg/L, and the lipid peroxidation of liver homogenate and mitochondria induced by auto-oxidation and induced-oxidation. The inhibiton exhibited concentration-dependent manner. Conclusion:It indicated that TCF could scavenge oxygen free radicals and was capable of prophylaeticly antagonizing against the oxidative injury by superoxide anion.
出处
《中药材》
CAS
CSCD
北大核心
2003年第5期338-340,共3页
Journal of Chinese Medicinal Materials
