摘要
为指导蜡质芽孢杆菌AT31防治根结线虫的田间使用技术,采用不同剂量进行室内盆栽试验,15倍液防效达55.69%,显著高于低剂量防效。蜡质芽孢杆菌AT31产品在使用前进行二次发酵,48~72h的发酵液中菌体数和芽孢数均在1.3×109CFU/m L以上,达到产品原液有效成分含量水平。二次发酵液的不同剂量防治根结线虫盆栽试验显示450L/hm2为田间适宜剂量,对根结线虫防效为66.67%。大棚田间试验表明蜡质芽孢杆菌AT31产品在田间使用前利用二次发酵的方法可以有效的控制根结线虫病害,防效达73.46%,与产品原液及噻唑膦10%颗粒剂防效相近。
In order to instruct the technology using the Bacillus cereus AT31 to prevent and control the root knot nematode, the experiment was conducted with different concentration in greenhouse. The result showed the control effect of 15 times dilution reached 55.69%, which is significantly higher than other lower dilutions. The number of thallus and spore content are both on the level of 1.3×109CFU/m L after 48 to 72 hours in Bacillus cereus AT31 with secondary fermentation, which reached the level of the a.i. content of the product solution. The optimum dose is 450L/hm2 with control effect 66.67%. The result showed, it is an efficient method to control root knot nematode using Bacillus cereus AT31 with secondary fermentation with control effect 73.46%,similar to the effect of original solution and Fosthiazate10% granules.
出处
《农药科学与管理》
CAS
2015年第10期52-57,共6页
Pesticide Science and Administration
基金
江苏省科技创新与成果转化(重大成果转化)资金(编号:BA2012059)
关键词
根结线虫
蜡质芽孢杆菌
二次发酵
药效试验
防治效果
root-knot nematode
bacillus cereus
secondary fermentation
efficacy trial
control effect
