摘要
从近年来由我国不同地区、不同宿主分离到的新城疫病毒 (NDV)野外分离株中 ,选取其中具有代表性的 1 6株 ,用 RT- PCR技术扩增其 F基因重要的功能区片段 ,进一步进行克隆和序列测定。参照国内外已发表的部分毒株的 F基因序列 ,构建了 5 9株 NDV的遗传进化树 ,分析其毒株间的遗传进化关系。序列分析表明 ,所扩增的目的片段长度为 5 35 bp,所分离的毒株在分裂位点的氨基酸顺序为 1 1 2 R- R- Q/ R- K/ R- R- F1 1 7,均相当于 NDV的强毒株。通过遗传进化树分析表明 ,1 6株分离株中有 1 3株为基因 型 NDV,说明目前基因 型 NDV所引起的新城疫在国内呈流行趋势。
Sixteen strains of APMV Ⅰ had been isolated from clinical cases during 1994 to 2000 in different parts of China.Their main function segment of Fusion gene(from 47 to 581 bp) were amplified by RT PCR and then cloned into pGEM R T vector.The sequence analysis showed that the length of the F gene segment were 535 bp.The amino acid sequence of the cleavage site region was 112 R R Q/R K/R R F 117 ,matching to virulent NDV strains.A phylogenetic tree based on obtained sequences of reference NDV strains was constructed.The results revealed that thirteen isolates belonged to genotype Ⅶ indicating the genotype Ⅶ NDV may be the major pathogen causing the epizootic of ND in recent years in China.
出处
《兽医大学学报》
CSCD
北大核心
2003年第3期218-221,共4页
关键词
新城疫病毒
流行现状
RT-PCR
基因
Newcastle disease virus
molecular epidemiology
RT PCR
genotype
