摘要
功能性红曲产品中的MonacolinK包括酸型和内酯型两种成分.采用HPLC法,色谱柱为ZORBAXSBC 18,5μm,250mm×4.6mm,柱温28℃,V(乙腈)∶V(0.1g/dL磷酸水溶液)=65∶35为流动相,紫外检测器波长为238nm,可同时对酸型和内酯型的MonacolinK进行定量检测,线性关系和重复性均满足要求.固体红曲样品可以用甲醇直接萃取,液态的红曲样品在甲醇萃取之前需调整pH值6～7,测定结果能反映原样品中两种形态MonacolinK的含量.色素对MonacolinK检测存在一定影响,但对于多数功能性红曲色价低、MonacolinK高的特点,这一影响作用较轻,可以忽略.
Highperformance liquid chromatography, using a ZORBAX SB C18 column(5 μm,250 mm×4.6 mm) and acetonitrile0.1 g/dL phosphate solution(63∶35) as mobile phase with detection at 238 nm was used to analyze the Monacolin K in functional red rice(or red yeast rice). Under such chromatographic condition, the two forms of Monacolin K (acid form and lactone form of Monacolin K) can be determined simultaneously. The method demonstrated good linear relationship and repeatability. Solid Monascus product samples could be measured after directly extracted by methanol, while liquid Monascus fermentation broth was extracted by methanol after adjusting the solution pH to 6～7. The results can show the real content of the two forms of Monacolin K in the samples. Pigments were found to effect the determination of Monacolin K. However, this effect can often be ignored when the pigment content is low and Monacolin K content is high.
出处
《无锡轻工大学学报（食品与生物技术）》
CSCD
北大核心
2003年第3期46-52,共7页
Journal of Wuxi University of Light Industry
