摘要
目的 :检测不育病人精浆胆固醇酯转运蛋白 (cholesterolestertransferprotein ,CETP)的含量 ,并探讨其与不育的关系。 方法 :随机选择 163例不育病人及 15例生育男性 ,行精液常规分析及精浆CETP浓度测定 ,其中5 5例不育病人同时测定了血清CETP含量。 结果 :不育病人及生育男性精浆CETP含量分别为 ( 2 .2 1± 1.2 3 )μg/L和 ( 1.40± 0 .45 ) μg/L ,两组间差异无显著性 (P >0 .0 5 ) ;在不育病人中 ,无精子症组 (n =2 9)、少弱精子症组(n =5 8)、少精子症组 (n =15 )、弱精子症组 (n =44 )及正常精子症组 (n =17)间CETP含量差异也无显著性 (P >0 .0 5 )。精浆平均CETP含量仅占血清的 1/ 10 0 0 ,不育病人精浆及其血清CETP含量间并无相关性 (r =0 .0 0 9,P>0 .0 5 )。 结论 :精浆CETP含量极低 ,与精子密度、活率的改变无关 。
Objectives: To detect the cholesterol ester transfer protein(CETP) levels in semen of infertile patients and evaluate the correlation between CETP in semial plasma and infertility. Methods: One hundred and sixty-three infertile patients and fifteen fertile males were selected randomly. The routine examination of ejaculates was fulfilled by computer aided semen analysis(CASA). The CETP levels in all seminal plasma samples and fifty-five serum samples were detected by ELISA method. Results: The CETP levels in infertile patients and fertile males were ( 2.21± 1.23) μg/L and ( 1.40± 0.45) μg/L, respectively. There were no significant differences between the two groups(P> 0.05). And there were no significant differences of CETP levels in seminal plasma among groups of azoospermia(n=29), oligoasthenozoospermia (n=58), oligospermia(n=15), asthenozoospermia(n=44) and normozoospermia(n=17) in the infertile patients(P> 0.05). The CETP in seminal plasma and serum were detected in 55 infertile patients, and there was no correlation between CETP levels in seminal plasma and serum using Spearman analysis(r= 0.009,P> 0.05). The mean CETP level in seminal plasma was almost 1/ 1 000 of that in serum. Conclusions: The CETP level in seminal plasma is extremely low and has no relation with the changes of sperm density or motility. It may ensure the integrity of sperm membrane before the sperm enters into female genital tract. Natl J Androl,2003,9(3):178-180
出处
《中华男科学杂志》
CAS
CSCD
2003年第3期178-180,共3页
National Journal of Andrology
关键词
胆固醇酯转运蛋白
精液
获能
不育
Cholesterol ester transfer protein
Semen
Capacitation
Male infertility
