摘要
目的一个稳定的人类正常口腔黏膜角化细胞体外培养体系对于研究组织工程化口腔黏膜构建及口腔黏膜上皮癌变及阻断有重要意义。研究体外培养的人正常口腔黏膜角化细胞的细胞生物学特性。方法通过测定细胞生长曲线及克隆形成率了解细胞生长基本规律及其增殖能力,并通过流式细胞仪检测细胞染色体倍性。结果细胞接种后第1～3天为静止生长期,第5～10天处于对数生长期,第12天后进入平台期。倍增时间为24～48h;接种密度为200/cm2时,培养细胞克隆形成率为0.979%。经流式细胞仪检测细胞染色体倍性结果表明原代及传代细胞均为二倍体细胞。结论体外连续培养的口腔黏膜角化细胞为正常上皮细胞,增殖能力良好。
Aim To study biologic specificities in culturing normal human oral keratinocytes in vitro.Methods The growth curve, cloning efficiency and flow cytometry were used to measure basic law, proliferative ability and chromatosome ploidy of cells.Results After cells inoculation, 1-3 days was latent phase, 5-10 days was logarithmic phase and stagnate phase was from 12 days. The doubling time was 24-48 hours. The cloning efficiency was 0.979%at the inoculating density of 200 cells/cm2. The cultured cells were diploid cells.Conclusion The cultured cells are the normal cells and the state of proliferation is well.
出处
《中国临床康复》
CSCD
2003年第11期1628-1629,共2页
Chinese Journal of Clinical Rehabilitation
基金
国家自然基金(39970791)
上海市教委(99XD14003)~~
