摘要
从北京、山东、河北、湖南、湖北、江西、安徽、广西等板栗主产区的腐烂栗实中分离出146个分离物,经鉴定归属于11个属。不同产区栗实的自然带菌情况各异,带菌量和带菌种类有所不同,南方板栗产区的病原菌种类多于北方产区。回接实验研究了其中分布较普遍的6个属优势菌的致病力,表明不同菌的致病力强弱有较大差异,在相同培养条件下,Rhizoctoria使种仁的发病程度最严重,致病力最强,Penicilliumsp.的致病力最弱。不同接种方式对种仁侵染率的影响不同,种仁有伤接种的侵染率最高,达90%以上,而种皮接种和无伤滚动浸涂接种则不会使种仁发病腐烂。研究中还发现,不同病原菌所致板栗腐烂的病斑特征有所不同。
One hundred fortysix isolates were obtained from samples of Chinese chestnut (Castanea mollissima) collected from eight different production areas in Beijing, Hebei, Shandong, Anhui, Jiangxi, Hubei, Hunan, Guangxi. They were designated as 11 genera, and the pathogenic ability of 6 popularly distributed genera of 11 was studied through reinoculation experiment. The results showed that the natural fungal flora in chestnut seeds were different among different production areas. The most popularly distributed fungi are Penicillium sp., Fusarium sp., Mucor sp., Dothiorella sp., Pestalotiopsis sp., Phomopsis sp. and Rhizoctonia sp.. Reinoculation experiment of several predominant fungi reflected that the pathogenic ability among different fungi has great diversity. At the same condition, the degree of a disease caused by Rhizoctoria sp. was the most deeply and the pathogenic ability was the strongest while the pathogenic ability of Penicillium sp. was the weakest among these 6 predominant fungi. The effect on the rot of the flesh of chestnut seeds was very different in different methods of inoculation. The incidence of a disease inoculated the flesh was higher than any other ways of inoculation and reached more than 90%. But the flesh did not rot by inoculating the peel of chestnut seeds, or rolling the whole sound seeds in the suspended solution of spores. From the reinoculation experiment by using a single fungus we can see that the characteristic of rotted spot caused by different pathogen microbe has great diversity.
出处
《林业科学研究》
CSCD
北大核心
2003年第3期284-288,共5页
Forest Research
基金
林业部重点项目"几种名特优果品贮藏保鲜技术"和国家计委重点科技攻关项目"经济林产品贮藏保鲜加工技术研究"(1997-2000)
关键词
板栗
病原菌
产区
种类
致病力
症状
chestnut
rot
pathogenic fungi
pathogenic ability
