摘要
目的 :研究谷氨酰胺对内毒素损伤的心肌的保护作用研究。方法 :取健康体重 (2 5 0± 30 )gSD雄性大鼠 2 2只随机分成 3组 ,正常对照组 (n =7) ,内毒素组 (n =7) ,谷氨酰胺 +内毒素组 (n =8) ,在Langendorff装置上用Krebs-Henseleit(KH)液对大鼠离体心脏行主动脉逆灌。连续记录心肌细胞单相动作电位 (MAP)、心肌收缩张力曲线 ,在相应的时点分别测定冠脉流出液中的超氧化物歧化酶 (SOD)活性和丙二醛 (MDA)含量。结果 :谷氨酰胺组对内毒素损伤引起的心肌细胞单相动作电位幅度减小及D2 0 延长、心肌张力降低、心率变慢等变化有明显的改善作用 ,同时可以明显逆转内毒素诱导的心肌SOD活性降低和MDA含量升高。结论 :谷氨酰胺对内毒素损伤的心肌有明显的保护作用 ,有机会成为一个有效防治内毒素性心肌损伤的药物。
AIM:To observe protective effect of L-glutamine on myocardial cell injury induced by endotoxin. METHODS:22 male SD rats,weighting(250±30)g,were randomly divided into three groups:control group( n= 7),endotoxin group( n= 7),glutamine/endotoxin group( n= 8). Heart were isolated from rats and perfused on Langendorff apparatus with Krebs- Henseleit(K-H)buffer(Saturation 95%O 2+5%CO 2)at a constant pressure(8.33 kPa)and temperature(37℃). The activity of superoxide dismutase (SOD)and malondialdehyde (MDA)content of efflux from coronary artery, monophasic action potential(MAP)and contractile force were measured at certain timepoints (0 min,20 min,50 min,80 min). RESULTS: Monophasic action potential and contractile force were markedly improved in Gln/ET group. The activity of SOD and MDA level in Gln/ET group restored closely to that in control group. CONCLUSION:L-gluta mine protects myocardial cells from injure induced by endotoxin.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2003年第6期765-768,共4页
Chinese Journal of Pathophysiology
基金
广东省重点科技攻关项目 (2KM0 4 70 3S)
广东省医学科研基金项目 (B2 0 0 2 0 6 6 )
暨南大学医学院科研基金资助项目 (2 0 0 113)
