摘要
利用根癌农杆菌 (Agrobacteriumtumefaciens)介导 ,首次将菜豆几丁质酶和烟草β 1 ,3 葡聚糖酶双价水解酶基因导入番茄品种A5 3(Lycopersiconescu lentumcv .A5 3)中 ,获得批量转基因再生植株。对外源基因的PCR和Southern杂交结果表明 ,外源基因已经整合到番茄基因组中 ,其拷贝数 1～ 8个不等。Northern杂交和卡那霉素喷施表明目的基因和标记基因均已得到表达 ,抗病性鉴定初步表明转基因植株对枯萎病的抗性显著提高。
The genes encoding bean chitinase and tobacco β 1, 3 glucanase were introduced into the tomato line A53 ( Lycopersicon esculentum cv.A53) via an Agrobacterium mediated transformation system. Transformants were obtained and confirmed by PCR and Southern blot analysis. The integration of one to eight copies of the transgenes into the tomato genome was shown. Transgenic plants obtained expressing the foreign genes showed resistance to Fusarium wilt disease.
出处
《植物生理与分子生物学学报》
CAS
CSCD
2003年第3期179-184,共6页
Journal Of Plant Physiology and Molecular Biology
基金
武汉市科技攻关项目 (No .992 0 0 2 0 45G)资助~~
