摘要
采用组织化学方法,AB-PAS染色,对人工养殖的1龄海湾扇贝(Argopecten irradians,壳长35～40 mm)进行显微观察,发现海湾扇贝唇瓣、口唇、胃、肠、直肠、晶杆囊和肝脏中均有粘液细胞分布,其中,内唇瓣的内表皮和外唇瓣的外表皮粘液细胞数量较多,其中Ⅱ型(AB-PAS染色呈蓝色)最多,Ⅳ型(蓝紫色)次之,Ⅲ型(紫红色)较少,Ⅰ型(红色)极少;内唇瓣的外表皮和外唇瓣的内表皮粘液细胞仅在每一横褶的皱褶处集中分布,多为Ⅱ型,另有少量Ⅳ型。口唇粘液细胞较多,排列比较紧密,Ⅱ型最多,ⅣJ型次之。胃粘液细胞数量很少,均为Ⅱ型。肠粘液细胞(Ⅱ型)较多,在肠上皮细胞较薄的区域分布密集。直肠粘液细胞(Ⅱ型)较多。晶杆囊粘液细胞(Ⅱ型)仅分布于局部。粘液细胞(Ⅱ型)在肝脏仅有少量分布于输出管壁和外上皮中。
The scallop Argopecten irradians (body length 35-40 mm) were observed and analyzed by using AB-PAS (alcian blue and periodic acid schiffs reaction) combined procedure. As a result, the mucous cells are distributed in all of the digestive organs including labial palp, mouth labia, stomach, intestines, rectum, crystalline sac and digestive diverticula. The mucous cells in the inner epithelia of inner labial palp and in the outer epithelia of outer labial palp are abundant and they are divided into four types. The number of the four types follows the order that; typeⅡ( blue in AB-PAS) > type Ⅳ (blue purple) > type Ⅲ ( purple red) > type Ⅰ (red). The mucous cells in the outer epithelia of inner labial palp and in the inner epithelia of outer labial palp are only distributed in folds, and most are in type Ⅱ and a few of them are in type Ⅳ. The mucous cells are rich in the mouth labia and arranged closely with a large number of type Ⅱand a few number of type Ⅳ. There are a few mucous cells in the epithelia of stomach and all of them are in type Ⅱ. The mucous cells (type Ⅱ ) are abundant in the epithelia of intestines and gather in the thin areas. The mucous cells (typeⅡ ) are also abundant in the epithelia of rectum, but only distributed in part regions of crystalline sac. There are a few mucous cells (type Ⅱ ) in the transport duct wall and outer epithelia of digestive diverticula.
出处
《中国水产科学》
CAS
CSCD
北大核心
2003年第3期254-257,共4页
Journal of Fishery Sciences of China
基金
国家"九七三"计划资助项目(G1999012005)
山东省中青年学术骨干资助项目
关键词
海湾扇贝
消化系统
粘液细胞
Argopecten irradians
digestive system
mucous cell
