摘要
目的:探索成年大鼠雪旺细胞的体外培养和纯化方法。方法:取成年大鼠背根神经节采用植块法培养,坐骨神经采用酶消化法分散培养。各分三组,第一组为阿糖胞苷处理组;第二组为免疫溶解处理后加垂体浸出液组;第三组为对照组。以活细胞计数和S-100标记相结合判断雪旺细胞增殖和纯化程度。结果:采用植块培养法至第2周末,雪旺细胞纯度在第一、二和三组分别为90％、97％和50％。分散培养至第18天,雪旺细胞纯度第一组94％,第二组大于97％,第三组80％。细胞数量第一组增加1.4倍,第二组增加5.1倍,第三组增加2.4倍。结论:采用免疫溶解处理后加垂体浸出液方法培养成年大鼠来源的雪旺细胞,可得到数量多纯度高的雪旺细胞。
Objective:To search for the methods of proliferation and purification of Schwann cells derived form adult rats. Methods:The desheathed dorsal root ganglions removed from adult rats were cultured with explant culture. The sciatic nerves were digested with enzymes, and then the isolated cells were culture in vitro. All cultured cells were divided into 3 groups,group 1 treated with cytosine arabinoside: group 2 treated with immunoselective method and pituitary extract; Group 3 as a control. The proliferation and purification were evaluated according to living cell count and S-100 immunocy-tochemistry stain. Results: At the end of second week in dorsal root ganglion culture, the purification of Schwann cells was 90% in group 1,97% in group 2 and 50% in group 3. When Schwann cells were dissociated from sciatic nerves and cultured to the 18th day, the purification of Schwann cells was 94% in group 1;more than 97% in group 2 and 80% in group 3. The number of Schwann cells increased 1.4 times in group 1,5.1 times in group 2 and 2.4 times in group 3. Conclusion: When the dorsal root ganglions and sciatic nerves derived from adult rats are treated with immunoselective method and pituitary extract stimulation, large amount of and high purified Schwann cells can be harvested.
出处
《解剖学杂志》
CAS
CSCD
北大核心
2003年第1期81-84,共4页
Chinese Journal of Anatomy
